Pressure Induced Structural Changes and Gas Diffusion Pathways in Monomeric Fluorescent Proteins Dissertation

(2013). Pressure Induced Structural Changes and Gas Diffusion Pathways in Monomeric Fluorescent Proteins . 10.25148/etd.FI13042320

thesis or dissertation chair

fiu authors

  • Bhandari, Yuba R

abstract

  • Fluorescent proteins (FPs) are extremely valuable biochemical markers which have found a wide range of applications in cellular and molecular biology research. The monomeric variants of red fluorescent proteins (RFPs), known as mFruits, have been especially valuable for in vivo applications in mammalian cell imaging. Fluorescent proteins consist of a chromophore caged in the beta-barrel protein scaffold. The photophysical properties of an FP is determined by its chromophore structure and its interactions with the protein barrel.

    Application of hydrostatic pressure on FPs results in the modification of the chromophore environment which allows a systematic study of the role of the protein-chromophore interactions on photophysical properties of FPs. Using Molecular Dynamics (MD) computer simulations, I investigated the pressure induced structural changes in the monomeric variants mCherry, mStrawberry, and Citrine. The results explain the molecular basis for experimentally observed pressure responses among FP variants. It is found that the barrel flexibility, hydrogen bonding interactions and chromophore planarity of the FPs can be correlated to their contrasting photophysical properties at vaious pressures.

    I also investigated the oxygen diffusion pathways in mOrange and mOrange2 which exhibit marked differences in oxygen sensitivities as well as photostability. Such computational identifications of structural changes and oxygen diffusion pathways are important in guiding mutagenesis efforts to design fluorescent proteins with improved photophysical properties.

publication date

  • March 28, 2013

keywords

  • chromophore
  • chromophore planarity
  • diffusion pathways
  • fluorescence
  • hydrogen bonding
  • parameterization
  • quenching

Digital Object Identifier (DOI)