Ovarian Cancer therapy combining the modalities of hyperthermia and chemotherapy Thesis

(2007). Ovarian Cancer therapy combining the modalities of hyperthermia and chemotherapy . 10.25148/etd.FI14060820

thesis or dissertation chair

fiu authors

  • Chowdhury, Mohd Raquibul

abstract

  • Ovarian is the leading cause of deaths in women diagnosed with gynecological malignancies. Current treatments include chemotherapy which may cause severe side effects. The purpose of this study was to investigate the combinational therapeutic effect of chemotherapy and hyperthermia to an ovarian cancer cell line and compare the effects of inducing hyperthermia systemically with localized hyperthermia. Cells were seeded in 96-well plates and subjected to different doses of chemotherapy agent; Doxorubicin (DOX). To simulate systemic hyperthermia the 96-well plate was incubated for 60 minutes at 43°C. To simulate localized hyperthermia, Indocyanine Green was added to the seeded cells in 96-well plates, after which the wells were irradiated by 808 nm NIR laser. SRB assay was used at the end of the study to measure cell proliferation. The results showed that cell proliferation was reduced 110% when DOX was combined with localized hyperthermia compared with DOX or systemic hyperthermia alone.

publication date

  • April 5, 2007

Digital Object Identifier (DOI)