This research aims to monitor workers’ physiological reactions in virtual construction scenario. With the objective of leveraging affective sensing technology in construction scenario, experiments with Galvanic Skin Response (GSR) was conducted in a 3D simulation developed based on a real construction site. The GSR results obtained from sensor were analyzed in order (i) to assess the feasibility of using virtual environment to generate real emotions, (ii) to examine the relation between questionnaires used to ask people about their experience and their physiological responses and (iii) to identify the factors that affect people’s emotional reactions in virtual environment. Subjects of the experimental group exhibited incoherent responses, as expected in experiments with human subjects. Based on the various reasons for this incoherence obtained from questionnaire part of the experiment, the potential in research for developing training methods with respect to workers’ physiological response capability was identified.