Cocaine in the Neuropathogenesis of HIV infection: Role of HDAC-2 Grant

abstract

  • Cocaine is a significant risk factor for contracting HIV-1 infection and subsequently developing AIDS. The proposed research is aimed to dissect the role of cocaine and HIV-associated epigenetic mechanisms in the development of HIV-associated neurocognitive disorders (HAND). In recent years, chromatin remodeling has emerged as an important regulatory mechanism for drug addiction and also in development of neurodegenerative disorders. In the current study, we will focus on HDAC2 since it is known to play a critical role in cognitive function by regulating the density of dendritic spines and influencing synaptic transmission in mature neurons. However, the role of HDAC2 in development of HAND is not elucidated yet in the context of cocaine. In our published and preliminary studies, we found that HIV-1 Tat protein and HIV-1 infection upregulate HDAC2 expression with concomitant downregulation of CREB and CamKIIa and 17 other genes that are known to regulate synaptic plasticity. Further we have shown for the first time that cocaine significantly upregulated HDAC2 gene expression and downregulated miR-155 and transfection with miR-155 significantly inhibited HIV infection. Accordingly, we hypothesize that cocaine acts a co-factor in neuropathogenesis of HIV infection by modulating HDAC2 expression in CNS cells leading to transcriptional repression of genes that regulate neuronal activity and synaptic plasticity. The specific goals are to determine: 1) whether cocaine in association with HIV-1 virus modulates HDAC2 which in turn negatively regulates synaptic plasticity/ memory genes in primary human microglia, hippocampal neurons and astrocytes thereby contributing to the progression of HAND; 2) whether inhibition of HDAC2 reverses the effects of cocaine and HIV-1 virus on neuronal plasticity genes and dendritic spine density and 3) can delivery of HDAC2 specific siRNA and miR-155 across the in vitro blood-brain barrier (BBB) model and in HIV-E SCID cocaine mouse model using transferrin coupled liposomal nanoformulation be explored as a therapeutic strategy for HAND. Thus, an understanding of cocaine and HIV-associated epigenetic changes and its implications in neuropathogenesis will have translational significance for therapeutic targeting and control of HAND in HIV-infected cocaine users.

date/time interval

  • March 1, 2013 - December 31, 2015

sponsor award ID

  • 1R01DA034547-01

local award ID

  • AWD000000003139

contributor

keywords

  • AIDS/HIV problem
  • Abbreviations
  • Acetylation
  • Acquired Immunodeficiency Syndrome
  • Animal Model
  • Astrocytes
  • Behavioral
  • Binding
  • Blood - brain barrier anatomy
  • CREB1 gene
  • Ca(2+)-Calmodulin Dependent Protein Kinase
  • Calcium
  • Cells
  • Central Nervous System Diseases
  • Clinical Research
  • Cocaine
  • Cocaine Abuse
  • Cocaine Users
  • Cognitive
  • Contracts
  • Coupled
  • Cyclic AMP Response Element
  • Dendritic Spines
  • Development
  • Disease
  • Disease Progression
  • Down-Regulation
  • Drug Addiction
  • Drug abuse
  • Electrical Resistance
  • Epigenetic Process
  • Gene Expression
  • Genes
  • Goals
  • Growth
  • HDAC2 gene
  • HIV
  • HIV Infections
  • HIV-1
  • Hippocampus (Brain)
  • Histone Deacetylation
  • Histones
  • Human
  • Impairment
  • In Vitro
  • Individual
  • Infection
  • Injection of therapeutic agent
  • Liposomes
  • Mediating
  • Memory
  • Memory impairment
  • Microglia
  • Modeling
  • Modification
  • Molecular
  • Motor
  • Neurocognitive
  • Neurodegenerative Disorders
  • Neuronal Plasticity
  • Neurons
  • Neuropathogenesis
  • Patients
  • Play
  • Predisposition
  • Proteins
  • Publishing
  • Rattus
  • Regulatory Element
  • Reporting
  • Research
  • Risk Factors
  • Role
  • Self Administration
  • Small Interfering RNA
  • Synaptic Transmission
  • Synaptic plasticity
  • Therapeutic
  • Therapeutic Intervention
  • Time
  • Transcriptional Regulation
  • Transfection
  • Transferrin
  • Transferrin Receptor
  • Trichostatin A
  • Up-Regulation
  • Valproic Acid
  • Virus
  • chromatin modification
  • chromatin remodeling
  • cocaine use
  • cognitive function
  • density
  • drug abuser
  • drug of abuse
  • gene repression
  • histone acetyltransferase
  • histone deacetylase 2
  • inhibitor/antagonist
  • mouse model
  • nanoformulation
  • nervous system disorder
  • nuclease
  • public health relevance
  • receptor mediated endocytosis
  • response
  • tat Protein
  • therapeutic target