Toxicant-induced synaptic dysfunction and neurotoxicity in Parkinson disease Grant

abstract

  • DESCRIPTION (provided by applicant): Our long term goal is to study the mechanisms of neurodegeneration induced by environmental toxicants, genetic mutations and potential gene-environment interactions to gain insights into the pathogenesis of Parkinson's disease (PD). Advances in the genetics of PD have highlighted the critical role of mitochondrial dynamics (fission / fusion / movement) in neuronal function and survival. However, because monogenic familial PD represents only a small fraction of PD cases, it is critical to determine whether perturbed mitochondrial dynamics also plays a role in the nigrostriatal damage induced by environmental neurotoxicants. Most of these exogenous toxic molecules cause mitochondrial dysfunction either directly by blocking mitochondrial respiration, or indirectly through oxidative stress. Based on our preliminary data, this proposal will utilize two complementary toxicant-based animal models of nigrostriatal neurodegeneration: A) The herbicide paraquat (PQ) induces cell death primarily through oxidative stress. B) The pesticide/insecticide rotenone directly inhibits mitochondrial function. We hypothesize that whether excessive mitochondrial fission and dysfunction is induced directly by blocking mitochondrial respiration (rotenone) or indirectly by oxidative stress (PQ), promoting mitochondrial fusion will attenuate pre-synaptic dysfunction and neurotoxicity seen in these animal models. In Aim 1, we will investigate the impact of promoting mitochondrial fusion in the PQ mouse and rotenone rat models. Because PQ does not induce striatal damage in regular mice, we will use our novel mutant mice with deletion of the organic cation transporter 3 (Oct3-/-) to create a PQ animal model with damage in both nigra and striatum, as well as to enhance relevance to human gene-environment interactions because OCT3 variants have been associated with PD. Small molecule and gene-based approaches will be used for manipulation of mitochondrial fission/fusion machinery. Both neurorestorative and neuroprotective effects of these strategies will be determined in animals with pre-existing lesions and with active neurodegeneration. Striatal mitochondrial function, evoked striatal dopamine release in freely moving animals, synaptic function using electrophysiology, motor function and the integrity of the nigrostriatal pathway will be analyzed. In Aim 2, we will investigate the mechanisms by which PQ and rotenone induce mitochondrial fission and why blocking this process is protective. Where relevant, both animal models and cell cultures will be used. A wide range of state-of-the art equipment/techniques such as 3-dimensional electron microscopy, laser capture microdissection, the Seahorse extracellular flux analyzer and amperometry will be used to quantify alterations in levels of proteins/genes of interest specifically in nigral dopaminergic neurons, mitochondrial trafficking/morphology/function and synaptic density/function. Accomplishment of these aims will provide critical information regarding how toxic insults impact nigrostriatal pathway through perturbed mitochondrial dynamics and offer insights into a potential novel therapeutic target for PD.

date/time interval

  • September 9, 2016 - June 30, 2019

sponsor award ID

  • 5R01ES022274-04

local award ID

  • AWD000000007203

contributor

  • Tieu, Kim   Principal Investigator   2016 - 2019

keywords

  • 3-Dimensional
  • Acute
  • Animal Model
  • Animals
  • Attenuated
  • Cell Count
  • Cell Culture Techniques
  • Cell Death
  • Complement
  • Complex
  • Corpus striatum structure
  • Cytosol
  • DNA Sequence Alteration
  • Data
  • Dominant-Negative Mutation
  • Dopamine
  • Dyes
  • Electron Microscopy
  • Electrophysiology (science)
  • Environment
  • Environmental Risk Factor
  • Epidemiology
  • Equilibrium
  • Equipment
  • Exposure to
  • Functional disorder
  • Gene Proteins
  • Genes
  • Genetic
  • Genetic Transcription
  • Genetic study
  • Genus Hippocampus
  • Goals
  • Grant
  • Herbicides
  • High Pressure Liquid Chromatography
  • Human
  • Immunoelectron Microscopy
  • Immunohistochemistry
  • Impairment
  • Individual
  • Injectable
  • Injection of therapeutic agent
  • Insecticides
  • Lesion
  • Light
  • Link
  • Location
  • Measures
  • Membrane Potentials
  • Meperidine
  • Methods
  • Microdialysis
  • Midbrain structure
  • Mitochondria
  • Mitochondrial Electron Transport Complex I
  • Modeling
  • Monitor
  • Morphology
  • Motor
  • Motor Activity
  • Movement
  • Mus
  • Mutant Strains Mice
  • Mutation
  • Nerve Degeneration
  • Neurons
  • Neurotoxins
  • OPA1 gene
  • Organelles
  • Organic Cation Transporter
  • Oxidation-Reduction
  • Oxidative Stress
  • Oxygen Consumption
  • Paraquat
  • Parkinson Disease
  • Parkinsonian Disorders
  • Pathogenesis
  • Patients
  • Pesticides
  • Pharmacology
  • Play
  • Process
  • Proteins
  • Rattus
  • Recombinant adeno-associated virus (rAAV)
  • Respiration
  • Risk
  • Rodent Model
  • Role
  • Rotenone
  • Serotyping
  • Shapes
  • Slice
  • Superoxides
  • Synapses
  • Synaptosomes
  • Techniques
  • Testing
  • Time
  • Toxic Environmental Substances
  • United States
  • Variant
  • Western Blotting
  • base
  • cytochrome c
  • density
  • disease diagnosis
  • dopaminergic neuron
  • epidemiology study
  • extracellular
  • gene environment interaction
  • genetic approach
  • in vivo
  • inhibitor/antagonist
  • insight
  • interest
  • laser capture microdissection
  • mitochondrial dysfunction
  • mitochondrial membrane
  • nervous system disorder
  • neuropathology
  • neurorestoration
  • neurotoxic
  • neurotoxicity
  • new therapeutic target
  • nigrostriatal pathway
  • novel
  • novel therapeutics
  • protein aggregation
  • public health relevance
  • small molecule
  • synaptic function
  • therapeutic target
  • tool
  • toxicant
  • trafficking
  • vector