Barbieri, Manuel
- Professor, Biological Sciences , College of Arts, Sciences & Education
Contact Info

Overview
overview
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The Barbieri research group is interested in the following two areas of research.
Endocytosis and Signal transduction
How the small GTP Rab5, is linked to the oncogene Ras, tyrosine receptor kinases and other intracellular signaling molecules. This pathway may provide a model for understanding how receptor ligand interaction regulates intracellular trafficking while simultaneously generating proliferative signals in normal and disease cells.
Phagocytosis and signal transduction
Internalization of pathogens into the phagocytic vacuoles is a critical component of the innate immune response. Following formation, the phagocytic vacuoles (i.e. phagosomes) interact with the endocytic compartment. This process (i.e. phagosome maturation) involves multiple fusion and fission events with the endomembrane system, which in turn, culminate with the formation of the phagolysosome. We are using the Fc receptor as model to determine the factors that initiate and coordinate the phagosome maturation process.
research interests
- Endocytosis and Signal transduction
Scholarly & Creative Works
selected publications
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Article
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2020Role of RIN1 on telomerase activity driven by EGF-Ras mediated signaling in breast cancer. EXPERIMENTAL CELL RESEARCH. 396.Full Text via DOI: 10.1016/j.yexcr.2020.112318 Web of Science: 000589411600014
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2019Clitoria ternatea Flower Petal Extract Inhibits Adipogenesis and Lipid Accumulation in 3T3-L1 Preadipocytes by Downregulating Adipogenic Gene Expression. MOLECULES. 24.Full Text via DOI: 10.3390/molecules24101894 Web of Science: 000470996600052
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2019Olive oil addition to the high-fat diet reduces methylglyoxal (MG-H1) levels increased in hypercholesterolemic rabbits. MEDITERRANEAN JOURNAL OF NUTRITION AND METABOLISM. 12.Full Text via DOI: 10.3233/MNM-180229 Web of Science: 000459858900002
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2018Dehydroleucodine inhibits mitotic clonal expansion during adipogenesis through cell cycle arrest. PHYTOTHERAPY RESEARCH. 32.Full Text via DOI: 10.1002/ptr.6089 Web of Science: 000440930900015
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2018Leucodine blocks mitotic clonal expansion during preadipoctye differentiation through cell cycle arrest. PHYTOCHEMISTRY LETTERS. 24.Full Text via DOI: 10.1016/j.phytol.2017.12.011 Web of Science: 000432718900016
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2018The 4-N-acyl and 4-N-alkyl gemcitabine analogues with silicon-fluoride-acceptor: Application to F-18-Radiolabeling. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY. 148:314-324.
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2017Biomedical Properties and Origins of Sesquiterpene Lactones, with a Focus on Dehydroleucodine. NATURAL PRODUCT COMMUNICATIONS. 12:995-1005.Web of Science: 000404832100039
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2016Dehydroleucodine inhibits tumor growth in a preclinical melanoma model by inducing cell cycle arrest, senescence and apoptosis. CANCER LETTERS. 372:10-23.
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2016Rin1 restores host phagocytic activity during invasion by Pseudomonas aeruginosa. JOURNAL OF MEDICAL MICROBIOLOGY. 65:351-361.Full Text via DOI: 10.1099/jmm.0.000235 Web of Science: 000377846700002
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2015Growth Factor Influences Rab5 Function in Breast CancerFull Text via DOI: 10.1096/fasebj.29.1_supplement.893.1
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2015A Novel Insight into Dehydroleucodine Mediated Attenuation of Pseudomonas aeruginosa Virulence Mechanism. BIOMED RESEARCH INTERNATIONAL.Full Text via DOI: 10.1155/2015/216097 Web of Science: 000365209800001
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2015Inhibitory effect of Clitoria ternatea flower petal extract on fructose-induced protein glycation and oxidation-dependent damages to albumin in vitro. BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE. 15.
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2014Volatile constituents of five species of Portlandia (Rubiaceae). JOURNAL OF ESSENTIAL OIL RESEARCH. 26:125-129.Full Text via DOI: 10.1080/10412905.2013.851039 Web of Science: 000331360700009
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2013Conversion of Aristolochic Acid I into Aristolic Acid by Reaction with Cysteine and Glutathione: Biological Implications. JOURNAL OF NATURAL PRODUCTS. 76:965-968.
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2013Phospholipase A(1): A novel virulence factor in Trypanosoma cruzi. MOLECULAR AND BIOCHEMICAL PARASITOLOGY. 187:77-86.
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2013Regulation of Rab5 Function during Phagocytosis of Live Pseudomonas aeruginosa in Macrophages. INFECTION AND IMMUNITY. 81:2426-2436.
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2013Saw palmetto ethanol extract inhibits adipocyte differentiation. JOURNAL OF NATURAL MEDICINES. 67:619-625.
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2012Aristolochic Acid I Metabolism in the Isolated Perfused Rat Kidney. CHEMICAL RESEARCH IN TOXICOLOGY. 25:130-139.
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2012Dehydroleucodine and dehydroparishin-B inhibit proliferation and motility of B16 melanoma cells. PHYTOCHEMISTRY LETTERS. 5:581-585.Full Text via DOI: 10.1016/j.phytol.2012.05.018 Web of Science: 000307596700038
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2012Effect of EGF-receptor tyrosine kinase inhibitor on Rab5 function during endocytosis. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS. 525:16-24.
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2012Secondary metabolite uptake by the Aristolochia-feeding papilionoid butterfly Battus polydamas. BIOCHEMICAL SYSTEMATICS AND ECOLOGY. 40:126-137.Full Text via DOI: 10.1016/j.bse.2011.10.006 Web of Science: 000299712700024
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2011Dehydroleucodin: a guaiane-type sesquiterpene lactone. ACTA CRYSTALLOGRAPHICA SECTION E-STRUCTURE REPORTS ONLINE. 67:O3470-U211.
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2011The effect of dehydroleucodine in adipocyte differentiation. EUROPEAN JOURNAL OF PHARMACOLOGY. 671:18-25.
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2009Functional determinants of ras interference 1 mutants required for their inhbitory activity on endocytosis. EXPERIMENTAL CELL RESEARCH. 315:820-835.
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2009Inhibition of early endosome fusion by Rab5-binding defective Ras interference 1 mutants. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS. 482:83-95.
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2009Intracellular Trafficking and Signaling: The Role of Endocytic Rab GTPase. TRAFFICKING INSIDE CELLS: PATHWAYS, MECHANISMS AND REGULATION. 405-418.Full Text via DOI: 10.1007/978-0-387-93877-6_19 Web of Science: 000270966400019
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2006Als2-deficient mice exhibit disturbances in endosome trafficking associated with motor behavioral abnormalities. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 103.Full Text via DOI: 10.1073/pnas.0510197103 Web of Science: 000238660400039
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2006Rab22a regulates the sorting of transferrin to recycling endosomesFull Text via DOI: 10.1128/MCB.26.7.2595-2614.2006
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2006Rab5-activating protein 6, a novel endosomal protein with a role in endocytosis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 340:967-975.
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2006Rin1 is a negative regulator of the IL3 receptor signal transduction pathways. ANTICANCER RESEARCH. 26:905-916.PMID: 16619486 Web of Science: 000236674800013
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2006Rin1 regulates insulin receptor signal transduction pathways. EXPERIMENTAL CELL RESEARCH. 312:1106-1118.
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2006Role of Rab5 in insulin receptor-mediated endocytosis and signaling. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS. 449:130-142.
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2004Role of Rab5 in EGF receptor-mediated signal transductionFull Text via DOI: 10.1078/0171-9335-00381
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1999Endosome fusion in living cells overexpressing GFP-rab5.Full Text via DOI: 10.1242/jcs.112.21.3667
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1999A Salmonella virulence protein that inhibits cellular traffickingFull Text via DOI: 10.1093/emboj/18.14.3924
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1998Protein kinase B/akt and Rab5 mediate Ras activation of endocytosisFull Text via DOI: 10.1074/jbc.273.31.19367
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1997Sequential actions of Rab5 and Rab7 regulate endocytosis in the Xenopus oocyteFull Text via DOI: 10.1083/jcb.136.6.1227
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1996Characterization of Rab5:Q79L-stimulated endosome fusionFull Text via DOI: 10.1006/abbi.1996.0047
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1995Myristoylation cannot functionally replace the isoprenylation of Rab5Full Text via DOI: 10.1006/abbi.1995.1070
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1992Trypanosoma cruzi: Inhibition of metacyclogenesis by mannoseFull Text via DOI: 10.1016/S0006-291X(05)81465-2
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Book
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2004Receptor tyrosine kinase signaling and trafficking - Paradigms revisited. Ed. 286.Full Text via DOI: 10.1007/978-3-540-69494-6_1
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Book Chapter
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2001Measurement of Rab5 protein kinase B/akt and regulation of Ras-activated endocytosis. 145-156.Full Text via DOI: 10.1016/S0076-6879(01)29075-2
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1995GTPases: Key regulatory components of the endocytic pathway. 223-234.Full Text via DOI: 10.1007/978-3-642-79547-3_14
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Conference
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2018Specific requirement for Ras interference 1 during cell migration. 242-242.Web of Science: 000505772700312
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1996
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Meeting Abstract
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2018EXPLORATION OF VERY LONG-RANGE LINKAGE DISEQUILIBRIUM WITHIN THE EXTENDED HLA REGION. HLA. 433-434.Web of Science: 000430290800236
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2017Synthesis of amino derivatives of dehydroleucodine and dehydroparishin-B as potential anti-proliferative against breast cancer and B16 melanoma cells. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.Web of Science: 000430569104714
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2017Antiproliferative Activity of Natural Compounds Isolated from Artemisia species.. MOLECULAR BIOLOGY OF THE CELL.Web of Science: 000426664303402
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2017
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2017Role of Small GTPases on differentiation of pre-adipocyte. MOLECULAR BIOLOGY OF THE CELL.Web of Science: 000426664302001
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2017SNPS CORRELATING WITH FUNCTIONAL HLA REGION EPITOPES AND SUPERTYPES OFFER INSIGHT INTO GWAS. HLA. 360-360.Web of Science: 000400973300048
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2016Dihydro derivatives of dehydroleucodine inhibit early adipogenesis. MOLECULAR BIOLOGY OF THE CELL.Web of Science: 000396047100059
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2016FUNCTIONAL ANNOTATIONS OF COMMON DISEASE MARKERS IN IMMUNE REGULATORY GENES. HLA. 215-215.Web of Science: 000383951500035
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2016Infection of Pseudomonas aeruginosa selectively affects human leukocyte antigen (HLA) gene expression.. MOLECULAR BIOLOGY OF THE CELL.Web of Science: 000396047100629
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2016Methylglyoxal levels increased in hypercholesterolemic rabbits. MOLECULAR BIOLOGY OF THE CELL.Web of Science: 000396046900551
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2016Rab5-Activating Protein 6 (RAP6) Activity in Adipogenesis. FASEB JOURNAL.Web of Science: 000406444003284
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2016Selective effect of Rin1 mutants on insulin signaling during pre-adipocyte differentiation.. MOLECULAR BIOLOGY OF THE CELL.Web of Science: 000396046900785
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2015A single amino acid change in Ras Interference 1 alters its function. MOLECULAR BIOLOGY OF THE CELL.Web of Science: 000209928500412
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2015RIN1 Mediated Rescue of Rab5 Activity: A Novel Insight on Pseudomonas-Phagosome Interaction. FASEB JOURNAL.Web of Science: 000361722705152
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2015Rab5-Activating Protein 6 (RAP6) is required for Rab5 activation upon insulin stimulation. MOLECULAR BIOLOGY OF THE CELL.Web of Science: 000209928500390
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2014Requirements of Rab5-activity, growth factor-dependent in highly invasive breast cancer cell lines.. MOLECULAR BIOLOGY OF THE CELL.Web of Science: 000352094103090
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2013Antimicrobial activity of dehydroleucodine on Pseudomonas aeruginosa growth and virulence. MOLECULAR BIOLOGY OF THE CELL.Web of Science: 000209348704383
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2013Rab5 function in breast cancer cells. MOLECULAR BIOLOGY OF THE CELL.Web of Science: 000209348700190
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2012Selective activation of Rab5 by distinct endocytic pathways.. MOLECULAR BIOLOGY OF THE CELL.Web of Science: 000209348607117
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2011Activation of Rab5 via Diverse Routes of Epidermal Growth Factor Receptor Endocytosis.. MOLECULAR BIOLOGY OF THE CELL.Web of Science: 000305505503192
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2011Inhibition of Rab5 activation by Exotoxin S of Pseudomonas aeruginosa.. MOLECULAR BIOLOGY OF THE CELL.Web of Science: 000305505504234
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Review
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2022Role of Selenium in Viral Infections with a Major Focus on SARS-CoV-2Full Text via DOI: 10.3390/ijms23010280 Web of Science: 000751390400001
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2020A hypothesis for a novel role of RIN1-the modulation of telomerase function by the MAPK signaling pathway. BIOCELL. 525-534.Full Text via DOI: 10.32604/biocell.2020.011407 Web of Science: 000604967300007
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2017Biomedical properties and origins of sesquiterpene lactones, with a focus on dehydroleucodine. 995-1005.Full Text via DOI: 10.1177/1934578x1701200638
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Works By Students
chaired theses and dissertations
advised honors theses
- Godieva, Victoria, Phyloextracts from Genus Artemisia: Controlling Survival and Melanogenesis of Murine Metastatic Cells 2017
- Florit, Daniela, An overview on Human Leukocyte Antigen (HLA) region gene expression during Pseudomonas aeruginosa infection 2015
- Gonzalez, Victor M., The Role of Ras Oncogene in Differentiation of 3T3-L1 Preadipocytes 2013
- Stefancin, Chad V., Antimicrobial effect of Artemisia species on Pseudomonas aeruginosa 2013
- Rivero, Nathalie, The Effect of Natural Products and Small Gtpases in Adipogenesis 2012
- Noriega, Manuela, The Expression of Rap6 in the Adult Mouse Brain and Early Mouse Embryonic Development 2010
- Murphy, Travis, Studying Key Amino Acids in the Function of Ras Interference 1 (Rinl) SH2 and Vps9 Domains 2009
Research
principal investigator on
- Functional Genomics of Disease-associated HLA Region Polymorphisms awarded by U.S. Department of Defense 2013 - 2016
- Effect of Clitoria ternatea flower petal extract on in vitro fat formation awarded by Thailand Research Fund 2014 - 2016
- Intracellular Receptor Trafficking awarded by General Medical Sciences 2008 - 2014
- ASCB MAC - Linkage Fellow: Manuel Barbieri, PhD awarded by General Medical Sciences 2011 - 2012
- ASCB MAC - 2009 Summer Research Experience awarded by American Society for Cell Biology 2009 - 2010
co-principal investigator on
- Functional Genomics of Disease-associated HLA Region Polymorphisms awarded by U.S. Department of Defense 2013 - 2016
subproject principal investigator on
- 2007-2008 MBRS Support of Continuous Research Excellence at FIU. awarded by General Medical Sciences 2007 - 2009
investigator on
- Community-Engaged Research on COVID-19 Testing Among Underserved and/or Vulnerable Populations Phase II awarded by Minority Health and Health Disparities 2022 - 2023
- Community-Engaged Research on COVID-19 Testing Among Underserved and/or Vulnerable Populations awarded by National Institute on Drug Abuse 2020 - 2023
Contact
full name
- Manuel Barbieri
visualizations
Recent publications and grants in Scholars@FIU
publication subject areas
- Biochemistry & Molecular Biology
- Biophysics
- Biotechnology & Applied Microbiology
- Cell Biology
- Chemistry
- Environmental Sciences & Ecology
- Evolutionary Biology
- Food Science & Technology
- General & Internal Medicine
- Hematology
- Immunology
- Infectious Diseases
- Integrative & Complementary Medicine
- Life Sciences & Biomedicine - Other Topics
- Microbiology
- Oncology
- Parasitology
- Pathology
- Pharmacology & Pharmacy
- Plant Sciences
- Research & Experimental Medicine
- Science & Technology - Other Topics
- Toxicology