Gu, Haiwei
- Associate Professor, Environmental Health Sciences , Robert Stempel College of Public Health and Social Work

Overview
overview
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The Gu research group focuses on biomarker discovery and metabolic mechanism studies using multi-omics approaches. For metabolomics, we are skilled in the development, optimization, and applications of mass spectrometry (MS) methods for both metabolite level measurements and metabolic flux analysis. We utilize a wide range of platforms, including LC- and GC-MS for untargeted aqueous profiling and lipidomics. We have also developed a number of targeted LC-MS/MS assays to detect panels of metabolites, including >400 identified metabolites from >35 metabolic pathways, as well as assays to interrogate bile acids, acyl carnitines, co-enzymes, and cardiolipins. Recent methods developed by Gu group include quantitative methods to measure metabolite concentrations, innovative metabolic flux analysis approaches, and ratio analysis methods for unknown identification. In addition, we have extensive experience with advanced multivariate statistical analysis methods.
Recently, we are combining metabolomics and other omics technologies in systems biology, including 16S rRNA sequencing, meta-transcriptomic sequencing, RNA-seq, and other molecular biology approaches. We are working closely with a number of biological researchers and clinical practitioners in various studies. We have been active in the identification of biomarkers for early disease detection, recurrence monitoring, and therapy prediction. We are also involved in mechanistic studies of cancer, aging, Alzheimer's disease, autism, heart diseases, obesity and diabetes, health disparities, as well as nutritional and environmental sciences. We have experience running large sample sets that incorporate a number of quality control measures to ensure reliability of large datasets. Our work in multi-omics is thus applied to a broad range of applications in both basic and clinical metabolic research.
research interests
- Metabolomics, microbiome, metabolism, metabolic flux analysis, nutrition, environmental sciences, Alzheimer's Disease
Scholarly & Creative Works
selected publications
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Article
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2022Identification of metabolic pathways underlying FGF1 and CHIR99021-mediated cardioprotectionFull Text via DOI: 10.1016/j.isci.2022.104447 Web of Science: 000828202700011
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2022Central Nervous System Metabolism in Autism, Epilepsy and Developmental Delays: A Cerebrospinal Fluid AnalysisFull Text via DOI: 10.3390/metabo12050371 Web of Science: 000803178300001
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2022Metabolites Associated With Fatigue and Physical Activity in Childhood CancerFull Text via DOI: 10.1177/10998004221085029 Web of Science: 000787877700001
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2022A Metabolomic Aging Clock Using Human Cerebrospinal FluidFull Text via DOI: 10.1093/gerona/glab212
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2022Microbiome and metabolome profiles of high screen time in a cohort of healthy college studentsFull Text via DOI: 10.1038/s41598-022-07381-3 Web of Science: 000763603800002
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2021Daily Vinegar Ingestion Improves Depression Scores and Alters the Metabolome in Healthy Adults: A Randomized Controlled TrialFull Text via DOI: 10.3390/nu13114020 Web of Science: 000724105100001
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2021Inositol serves as a natural inhibitor of mitochondrial fission by directly targeting AMPKFull Text via DOI: 10.1016/j.molcel.2021.08.025 Web of Science: 000701769800011
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2021A Metabolomic Analysis of the Sex-Dependent Hispanic ParadoxFull Text via DOI: 10.3390/metabo11080552 Web of Science: 000689402900001
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2021NAD(+) Redox Imbalance in the Heart Exacerbates Diabetic CardiomyopathyFull Text via DOI: 10.1161/CIRCHEARTFAILURE.120.008170 Web of Science: 000685475000005
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2021Early Breast Cancer Detection Using Untargeted and Targeted MetabolomicsFull Text via DOI: 10.1021/acs.jproteome.1c00019 Web of Science: 000661127000011
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2021Hypoxia promotes erythroid differentiation through the development of progenitors and proerythroblastsFull Text via DOI: 10.1016/j.exphem.2021.02.012 Web of Science: 000646229000005
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2021ApoE4 Impairs Neuron-Astrocyte Coupling of Fatty Acid MetabolismFull Text via DOI: 10.1016/j.celrep.2020.108572 Web of Science: 000605650500005
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2020Comprehensive Isotopic Targeted Mass Spectrometry: Reliable Metabolic Flux Analysis with Broad CoverageFull Text via DOI: 10.1021/acs.analchem.0c01767 Web of Science: 000568657600033
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2020Gut Microbiome Critically Impacts PCB-induced Changes in Metabolic Fingerprints and the Hepatic Transcriptome in MiceFull Text via DOI: 10.1093/toxsci/kfaa090 Web of Science: 000593286700014
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2020Metabolite releasing polymers control dendritic cell function by modulating their energy metabolismFull Text via DOI: 10.1039/d0tb00790k Web of Science: 000542474800002
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2020Triple Negative Breast Cancer Detection Using LC-MS/MS Lipidomic ProfilingFull Text via DOI: 10.1021/acs.jproteome.0c00038 Web of Science: 000538772300017
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2020Identifying Significant Metabolic Pathways Using Multi-Block Partial Least-Squares AnalysisFull Text via DOI: 10.1021/acs.jproteome.9b00793 Web of Science: 000530084700008
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2020Targeting the pregnane X receptor using microbial metabolite mimicryFull Text via DOI: 10.15252/emmm.201911621 Web of Science: 000526640800009
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2019Metabolomics Test Materials for Quality Control: A Study of a Urine Materials SuiteFull Text via DOI: 10.3390/metabo9110270 Web of Science: 000501174700034
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2019Influence of Storage Conditions and Preservatives on Metabolite Fingerprints in UrineFull Text via DOI: 10.3390/metabo9100203 Web of Science: 000494273300011
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2019Plasma metabolomics profiles suggest beneficial effects of a low-glycemic load dietary pattern on inflammation and energy metabolismFull Text via DOI: 10.1093/ajcn/nqz169 Web of Science: 000488198500025
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2019Tryptophan Metabolites in Irritable Bowel Syndrome: An Overnight Time-course StudyFull Text via DOI: 10.5056/JNM19042
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2019Polybrominated Diphenyl Ethers and Gut Microbiome Modulate Metabolic Syndrome-Related Aqueous Metabolites in MiceFull Text via DOI: 10.1124/dmd.119.086538 Web of Science: 000480438000011
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2019Coccidioidomycosis Detection Using Targeted Plasma and Urine Metabolic ProfilingFull Text via DOI: 10.1021/acs.jproteome.9b00100 Web of Science: 000474795500010
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2019Combining NMR and MS with Chemical Derivatization for Absolute Quantification with Reduced Matrix EffectsFull Text via DOI: 10.1021/acs.analchem.8b05611 Web of Science: 000462098300041
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2019Biodiversity and dynamics of cyanobacterial communities during blooms in temperate lake (Harsha Lake, Ohio, USA)Full Text via DOI: 10.1016/j.hal.2018.12.006 Web of Science: 000465052600002
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2019Metabolic profiling identifies phospholipids as potential serum biomarkers for schizophreniaFull Text via DOI: 10.1016/j.psychres.2018.12.008 Web of Science: 000460994400003
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2019Breast cancer detection using targeted plasma metabolomicsFull Text via DOI: 10.1016/j.jchromb.2018.11.029 Web of Science: 000456893100004
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2019Loss of SETD2 Induces a Metabolic Switch in Renal Cell Carcinoma Cell Lines toward Enhanced Oxidative PhosphorylationFull Text via DOI: 10.1021/acs.jproteome.8b00628 Web of Science: 000455285900029
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2018Salivary metabolite profiling distinguishes patients with oral cavity squamous cell carcinoma from normal controlsFull Text via DOI: 10.1371/journal.pone.0204249 Web of Science: 000445626400097
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2018Chronic kidney disease attenuates the plasma metabolome response to insulinFull Text via DOI: 10.1172/jci.insight.122219 Web of Science: 000442534200019
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2018PBDEs Altered Gut Microbiome and Bile Acid Homeostasis in Male C57BL/6 MiceFull Text via DOI: 10.1124/dmd.118.081547 Web of Science: 000439231300019
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2018Alterations of eicosanoids and related mediators in patients with schizophreniaFull Text via DOI: 10.1016/j.jpsychires.2018.04.002 Web of Science: 000438660500025
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2018Circulating bile acids in healthy adults respond differently to a dietary pattern characterized by whole grains, legumes and fruits and vegetables compared to a diet high in refined grains and added sugars: A randomized, controlled, crossover feeding studyFull Text via DOI: 10.1016/j.metabol.2018.02.006
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2018Multiplatform Metabolomics Investigation of Antiadipogenic Effects on 3T3-L1 Adipocytes by a Potent DiarylheptanoidFull Text via DOI: 10.1021/acs.jproteome.8b00028 Web of Science: 000434367400009
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2015Targeted metabolic profiling of wounds in diabetic and nondiabetic miceFull Text via DOI: 10.1111/wrr.12299
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2015Metabolomics method to comprehensively analyze amino acids in different domainsFull Text via DOI: 10.1039/c4an02386b
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2015Aggression is associated with aerobic glycolysis in the honey bee brain1Full Text via DOI: 10.1111/gbb.12201
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2014Colorectal cancer detection using targeted serum metabolic profilingFull Text via DOI: 10.1021/pr500494u
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2013RAMSY: Ratio analysis of mass spectrometry to improve compound identificationFull Text via DOI: 10.1021/ac4019268
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Book Chapter
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2021Metabolomics Analysis of Viral Therapeutics. 179-197.Full Text via DOI: 10.1007/978-1-0716-1012-1_10 Web of Science: 000683424200011
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Conference
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2020Pretreatment serum metabolome predicts PFS in first-line trastuzumab-treated metastatic breast cancerFull Text via DOI: 10.1158/1538-7445.SABCS19-P4-10-25 Web of Science: 000527012502164
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2018Xenobiotic-Sensing Nuclear Receptors Regulate Bile Acid Homeostasis in a Gut Microbiota-Dependent Manner. 1056A-1057A.Web of Science: 000446020503079
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Other Scholarly Work
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2019Mass Spectral Similarity Networking and Gas-Phase Fragmentation Reactions in the Structural Analysis of Flavonoid Glycoconjugates (vol 91, pg 10413, 2019). 14780-14781.Full Text via DOI: 10.1021/acs.analchem.9b04825 Web of Science: 000498280100083
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Preprint
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2021Increased Glycolysis Underlies FGF1 and CHIR99021 Mediated CardioprotectionFull Text via DOI: 10.2139/ssrn.3962654
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Review
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2012Metabolic profiling: Are we en route to better diagnostic tests for cancer?. 1207-1210.Full Text via DOI: 10.2217/fon.12.113
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2012Direct analysis of biological samples using extractive electrospray ionization mass spectrometry (EESI-MS). 2145-2153.Full Text via DOI: 10.1007/s00216-012-5874-1
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2012Nuclear magnetic resonance spectroscopy and mass spectrometry-based metabolomics for cancer diagnosis. 1933-1937.Full Text via DOI: 10.1016/S1872-2040(11)60594-X
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2008Metabolomics-based methods for early disease diagnostics. 617-633.Full Text via DOI: 10.1586/14737159.8.5.617
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Research
principal investigator on
Contact
full name
- Haiwei Gu
Identifiers
ORCID iD
- https://orcid.org/0000-0002-7598-5022 (confirmed)