
Overview
overview
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My primary research interest focuses on single molecule cellular biophysics. I have expertise, experience, motivation and necessary skills to successfully carry out the proposed research. I am a biophysicist by training and have expertise in condensed matter physics, physical chemistry and biophysics. I have worked on electron transport through single molecule between two metal electrodes, the mass transport
of small molecules through nanopores and have applied these techniques to develop the next generation DNA sequencing techniques. At FIU, my group aims to develop novel single molecule/single cell techniques for the fundamental understanding of important biology problems. We are developing nanopore and nanoelectrode based hybrid methods with the integration of electrical, electrochemical and optical approaches to achieve single molecule level sensing and imaging in a single cell (ref. 1). They have been developed both on microfabricated chip platform and scanning ionic conductance microscopy (SICM)/inverted optical microscopy platforms. We are applying these novel techniques in drug delivery using synthetic nanoparticles, bioelectricity and cancer researches.
I have collaborated with PI since joining at FIU. We have used a scanning probe microscopy technique, SICM, to study the cell surfaces in response to various polymer nanoparticles. We have found that the cell surface morphologies are significantly dependent on the physical properties of CPNs (ref. 2). I have leadership and management skills for the proposed project. In the past five years at FIU, I have assembled a research team with postdoc, graduates and undergraduates. Currently I am conducting two NSF projects including a NSF CAREER award as a PI to develop novel nanopore/nanoelectrode probes in both microchip and microscope based platforms for extracellular detection and imaging. I had experience managing grant (e.g. staffing, research directions, budget), supervising graduate students, collaborating with other PIs and collaborators, and publishing research results. I have experience in supervising undergraduate and graduate students in research. I currently have a group with one postdoc and four PhD students. The current graduate students have made steady progress and several manuscripts have been published in peer-reviewed journals. I also provided research opportunities for more than ten undergraduates.
In summary, I have necessary knowledge and experience to conduct the proposed research activities. I strongly believe that studies of the endocytosis mechanism of nanoparticles will help us design and develop efficient drug delivery systems for therapeutics
research interests
- Dr. He studies nanoscale materials and systems, ranging from nanostructured materials to small organic molecule, biomolecules and polymers, individually or at small quantity. He’s group also probes or applies these nanoscale materials in controlled and confined nanoscale environments and/or in complex cellular and tissue environments. One goal of the research is to discover and understand new physics, chemistry or biology phenomena, and properties and functions at nanoscale. The other goal is to transfer the research discoveries into practical devices and tools, and to new applications that are relevant to promoting health and combating disease. To achieve the goal, his group builds and develops sophisticated instruments to integrate electrical, electrochemical and optical techniques. They also utilize various top-down and bottom-up micro/nano fabrication techniques to fabricate novel devices.
Scholarly & Creative Works
selected publications
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Article
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2022Probing the Intermediates of Catalyzed Dehydration Reactions of Primary Amide to Nitrile in Plasmonic JunctionsFull Text via DOI: 10.1021/acscatal.2c01793 Web of Science: 000819464100001
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2022Potentiometric and SERS Detection of Single Nanoparticle Collision Events on a Surface Functionalized Gold NanoelectrodeFull Text via DOI: 10.1149/1945-7111/ac6245 Web of Science: 000780481500001
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2022Detecting Individual Proteins and Their Surface Charge Variations in Solution by the Potentiometric Nanoimpact MethodFull Text via DOI: 10.1021/acssensors.1c02385 Web of Science: 000746813600001
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2021Pigmentation Affects Elastic Fiber Patterning and Biomechanical Behavior of the Murine Aortic ValveFull Text via DOI: 10.3389/fcvm.2021.754560 Web of Science: 000743549800001
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2021Gauging surface charge distribution of live cell membrane by ionic current change using scanning ion conductance microscopyFull Text via DOI: 10.1039/d1nr05230f Web of Science: 000722657900001
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2021Development of multifunctional nanopipettes for controlled intracellular delivery and single-entity detectionFull Text via DOI: 10.1039/d1fd00057h Web of Science: 000728669100001
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2021Effect of Electrical Stimulation on Spontaneously Beating Dynamics of Cardiac Tissues: An Analysis Using Digital Image CorrelationFull Text via DOI: 10.1002/admt.202100669 Web of Science: 000680276300001
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2021Developing Longer-Lived Single Molecule Junctions with a Functional Flexible ElectrodeFull Text via DOI: 10.1002/smll.202101911 Web of Science: 000675531100001
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2021Direct Observation of Amide Bond Formation in a Plasmonic Nanocavity Triggered by Single Nanoparticle CollisionsFull Text via DOI: 10.1021/jacs.1c02426 Web of Science: 000672592000009
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2021Detecting Individual Bond Switching within Amides in a Tunneling JunctionFull Text via DOI: 10.1021/acs.nanolett.1c01882 Web of Science: 000668003400067
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2021Highly sensitive detection of DNA damage in living cells by SERS and electrochemical measurements using a flexible gold nanoelectrodeFull Text via DOI: 10.1039/d1an00220a Web of Science: 000637210700023
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2021Effective Electrochemical Modulation of SERS Intensity Assisted by Core-Shell NanoparticlesFull Text via DOI: 10.1021/acs.analchem.0c04398 Web of Science: 000634562600012
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2021Sequencing of 53,831 diverse genomes from the NHLBI TOPMed ProgramFull Text via DOI: 10.1038/s41586-021-03205-y
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2020Simultaneous mapping of nanoscale topography and surface potential of charged surfaces by scanning ion conductance microscopyFull Text via DOI: 10.1039/d0nr04555a Web of Science: 000581650100016
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2020Observing dynamic molecular changes at single-molecule level in a cucurbituril based plasmonic molecular junctionFull Text via DOI: 10.1039/d0nr03360j Web of Science: 000561429100043
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2020Reliably Probing the Conductance of a Molecule in a Cavity via van der Waals ContactsFull Text via DOI: 10.1021/acs.jpcc.0c02411 Web of Science: 000555507000051
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2020Dynamic single-cell intracellular pH sensing using a SERS-active nanopipetteFull Text via DOI: 10.1039/d0an00838a Web of Science: 000548664800012
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2020Detection of Secretion of Exosomes from Individual Cell in Real-Time by Multifunctional Nanoelectrode-Nanopore NanopipettesFull Text via DOI: 10.1016/S1872-2040(20)60029-9 Web of Science: 000539012600001
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2020Electrochemical Enrichment and Raman Spectroscopy Detection of Dopamine and 5-Hydroxytryptamine by Gold NanoelectrodeFull Text via DOI: 10.19756/j.issn.0253-3820.191722 Web of Science: 000539012600009
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2020Long-Lived Gold Single-Atom Junctions Formed by a Flexible Probe for Scanning Tunneling Microscopy ApplicationsFull Text via DOI: 10.1021/acsanm.0c00161 Web of Science: 000529206200031
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2020The fabrication of a gold nanoelectrode-nanopore nanopipette for dopamine enrichment and multimode detectionFull Text via DOI: 10.1039/c9an01990a Web of Science: 000528803100034
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2020Differentiation of metallic and dielectric nanoparticles in solution by single-nanoparticle collision events at the nanoelectrodeFull Text via DOI: 10.1088/1361-6528/ab4445 Web of Science: 000504906300003
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2019High precision measurement of Compton scattering in the 5 GeV regionFull Text via DOI: 10.1016/j.physletb.2019.134884 Web of Science: 000488071200030
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2019Modulating and probing the dynamic intermolecular interactions in plasmonic molecule-pair junctionsFull Text via DOI: 10.1039/c9cp02030f Web of Science: 000477705800047
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2019Facile Fabrication of Gold Functionalized Nanopipette for Nanoscale Electrochemistry and Surface Enhanced Raman SpectroscopyFull Text via DOI: 10.1016/S1872-2040(19)61177-1 Web of Science: 000485258700003
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2019Electrospun PW12Ni5O43.5(isogenous) nanocomposites for highly efficient hydrogen evolution reactionFull Text via DOI: 10.1088/2053-1591/ab11bc Web of Science: 000463211800015
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2019Modulating Nanoparticle Translocation by Surface Chemistry of Gold NanoporesFull Text via DOI: 10.1016/S1872-2040(19)61174-6 Web of Science: 000473647100002
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2019Electrospun SiO2/WO3/NiWO4 decorated carbon nanofibers for an efficient electrocatalytic hydrogen evolutionFull Text via DOI: 10.1080/1536383X.2019.1609950 Web of Science: 000468686300001
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2019Mo2C-Ni modified carbon microfibers as an effective electrocatalyst for hydrogen evolution reaction in acidic solutionFull Text via DOI: 10.1016/j.jcis.2019.02.064 Web of Science: 000462416900032
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2019Numerical simulations of cell flow and trapping within microfluidic channels for stiffness based cell isolationFull Text via DOI: 10.1016/j.jbiomech.2019.01.010 Web of Science: 000461725000006
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2018Probing Dynamic Events of Dielectric Nanoparticles by a Nanoelectrode-Nanopore NanopipetteFull Text via DOI: 10.1002/celc.201800163 Web of Science: 000448518500025
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2018Cucurbituril mediated single molecule detection and identification via recognition tunnelingFull Text via DOI: 10.1088/1361-6528/aacb63 Web of Science: 000436960000001
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2018Monitoring the Dynamic Process of Formation of Plasmonic Molecular Junctions during Single Nanoparticle CollisionsFull Text via DOI: 10.1002/smll.201704164 Web of Science: 000430186600021
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2017Electrospun P-Mo-W-Ni multicomponent composite oxides for hydrogen evolution reactionFull Text via DOI: 10.1088/2053-1591/aa8d5d Web of Science: 000413093400007
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2017Quantum dot light emitting devices for photomedical applications.Full Text via DOI: 10.1002/jsid.543
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2016Simultaneous Ionic Current and Potential Detection of Nanoparticles by a Multifunctional NanopipetteFull Text via DOI: 10.1021/acsnano.6b06307 Web of Science: 000391079700069
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2016Electrospun MnCo2O4 nanofibers for efficient hydrogen evolution reactionFull Text via DOI: 10.1088/2053-1591/3/9/095018 Web of Science: 000385423000004
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2016Scanning Ion Conductance Microscopic Study for Cellular Uptake of Cationic Conjugated Polymer NanoparticlesFull Text via DOI: 10.1002/mabi.201500320 Web of Science: 000374262100012
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2016Carbon Nanotube Based Nanopore and Nanofluidic Devices Towards Sensing ApplicationsFull Text via DOI: 10.2174/1573413712666151120222819 Web of Science: 000390318900005
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2015Ambient Filtration Method To Rapidly Prepare Highly Conductive, Paper-Based Porous Gold Films for Electrochemical BiosensingFull Text via DOI: 10.1021/acsami.5b09612 Web of Science: 000366873900005
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2015Scanning Ion Conductance Microscopy for Single Cell Imaging and AnalysisFull Text via DOI: 10.1166/asem.2015.1812
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2015Note: Model identification and analysis of bivalent analyte surface plasmon resonance dataFull Text via DOI: 10.1063/1.4933318 Web of Science: 000364405300073
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2015Observation of the rare B-s(0)->mu(+)mu(-) decay from the combined analysis of CMS and LHCb dataFull Text via DOI: 10.1038/nature14474 Web of Science: 000355543400030
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2015Analyzing surface plasmon resonance data: Choosing a correct biphasic model for interpretationFull Text via DOI: 10.1063/1.4914027 Web of Science: 000352201400063
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2015Tunnelling current recognition through core-satellite gold nanoparticles for ultrasensitive detection of copper ionsFull Text via DOI: 10.1039/c4cc09451d Web of Science: 000349323500040
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2014Improved charge transport of Nb-doped TiO2 nanorods in methylammonium lead iodide bromide perovskite solar cellsFull Text via DOI: 10.1039/c4ta02635g Web of Science: 000344598700007
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2014Quantitative study of protein-protein interactions by quartz nanopipettesFull Text via DOI: 10.1039/c4nr02964j Web of Science: 000340520500046
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2013Surface modification of graphene nanopores for protein translocationFull Text via DOI: 10.1088/0957-4484/24/49/495102 Web of Science: 000328215300002
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2013Self-assembly of core-satellite gold nanoparticles for colorimetric detection of copper ionsFull Text via DOI: 10.1016/j.aca.2013.09.036 Web of Science: 000326684000015
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2013Fabrication of Core-Shell Nanoparticles via Controlled Aggregation of Semiflexible Conjugated Polymer and Hyaluronic AcidFull Text via DOI: 10.1021/ma400996y Web of Science: 000323193300063
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2013Optical and Electrical Detection of Single-Molecule Translocation through Carbon NanotubesFull Text via DOI: 10.1021/nn3050598 Web of Science: 000314082800073
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2012DNA translocating through a carbon nanotube can increase ionic currentFull Text via DOI: 10.1088/0957-4484/23/45/455107 Web of Science: 000310579200007
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2012Mass transport through vertically aligned large diameter MWCNTs embedded in paryleneFull Text via DOI: 10.1088/0957-4484/23/45/455101 Web of Science: 000310579200001
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2012Solution Synthesis of Ultrathin Single-Crystalline SnS Nanoribbons for Photodetectors via Phase Transition and Surface ProcessingFull Text via DOI: 10.1021/nn302504p Web of Science: 000306673800050
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2012Chemical recognition and binding kinetics in a functionalized tunnel junctionFull Text via DOI: 10.1088/0957-4484/23/23/235101 Web of Science: 000305160400001
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2012Electronic sensitivity of a single-walled carbon nanotube to internal electrolyte compositionFull Text via DOI: 10.1088/0957-4484/23/8/085203 Web of Science: 000300173200006
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2012Insulated gold scanning tunneling microscopy probes for recognition tunneling in an aqueous environmentFull Text via DOI: 10.1063/1.3673640 Web of Science: 000300594900060
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2011Gap Distance and Interactions in a Molecular Tunnel JunctionFull Text via DOI: 10.1021/ja2067737 Web of Science: 000295193700031
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2011Origin of Giant Ionic Currents in Carbon Nanotube ChannelsFull Text via DOI: 10.1021/nn202115s Web of Science: 000295187400058
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2011Electronic Sensitivity of Carbon Nanotubes to Internal Water WettingFull Text via DOI: 10.1021/nn200251z Web of Science: 000289742100081
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2010Recognition Tunneling Measurement of the Conductance of DNA Bases Embedded in Self-Assembled MonolayersFull Text via DOI: 10.1021/jp104792s Web of Science: 000284738900018
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2010Identifying single bases in a DNA oligomer with electron tunnellingFull Text via DOI: 10.1038/NNANO.2010.213 Web of Science: 000285047100014
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2010Translocation events in a single-walled carbon nanotubeFull Text via DOI: 10.1088/0953-8984/22/45/454112 Web of Science: 000283651400014
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2010Electronic Signatures of all Four DNA Nucleosides in a Tunneling GapFull Text via DOI: 10.1021/nl1001185 Web of Science: 000275278200056
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2010Translocation of Single-Stranded DNA Through Single-Walled Carbon NanotubesFull Text via DOI: 10.1126/science.1181799 Web of Science: 000273395400030
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2009Tunnel conductance of Watson-Crick nucleoside-base pairs from telegraph noiseFull Text via DOI: 10.1088/0957-4484/20/18/185102 Web of Science: 000265252900002
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2009Tunnelling readout of hydrogen-bonding-based recognitionFull Text via DOI: 10.1038/NNANO.2009.48 Web of Science: 000266220800012
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2009A hydrogen-bonded electron-tunneling circuit reads the base composition of unmodified DNAFull Text via DOI: 10.1088/0957-4484/20/7/075102 Web of Science: 000262786100002
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2008Charge transport in mesoscopic conducting polymer wiresFull Text via DOI: 10.1088/0953-8984/20/37/374120 Web of Science: 000258734700022
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2008Transverse tunneling through DNA hydrogen bonded to an electrodeFull Text via DOI: 10.1021/nl801646y Web of Science: 000258440700072
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2007Identification of DNA basepairing via tunnel-current decayFull Text via DOI: 10.1021/nl0726205 Web of Science: 000251581600055
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2007Length dependence of charge transport in oligoanilinesFull Text via DOI: 10.1063/1.2472758 Web of Science: 000244249800039
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2006Electrochemical origin of voltage-controlled molecular conductance switchingFull Text via DOI: 10.1021/ja0635433 Web of Science: 000242021700047
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2006Redox-gated electron transport in electrically wired ferrocene moleculesFull Text via DOI: 10.1016/j.chemphys.2006.02.022 Web of Science: 000238992400014
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2006Conductance of single alkanedithiols: Conduction mechanism and effect of molecule-electrode contactsFull Text via DOI: 10.1021/ja057316x Web of Science: 000235452200056
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2006Measuring single molecule conductance with break junctionsFull Text via DOI: 10.1039/b508434m Web of Science: 000235543600010
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2005On the mechanism of negative differential resistance in ferrocenylundecanethiol self-assembled monolayersFull Text via DOI: 10.1021/ja0532279 Web of Science: 000231605900027
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2005Switching of a photochromic molecule on gold electrodes: single-molecule measurementsFull Text via DOI: 10.1088/0957-4484/16/6/012 Web of Science: 000230144700012
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2005A molecular switch based on potential-induced changes of oxidation stateFull Text via DOI: 10.1021/nl0478474 Web of Science: 000227563700018
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2005Electronic decay constant of carotenoid polyenes from single-molecule measurementsFull Text via DOI: 10.1021/ja043279i Web of Science: 000226843900025
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Book Chapter
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2016Nano (Evanescent-Wave)-Particle Image Velocimetry. 2293-2293.Full Text via DOI: 10.1007/978-94-017-9780-1_100636
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2012Nano (Evenescent-Wave)-Particle Image Velocimetry. 1485-1485.Full Text via DOI: 10.1007/978-90-481-9751-4_100475
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Conference
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2019
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2019Nanopore-Nanoelectrode for Potential Sensing of Single Nanoparticle Collision Events. 444A-445A.Full Text via DOI: 10.1016/j.bpj.2018.11.2395 Web of Science: 000460779802234
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2018Analysis of MOOC Learning Rhythms. 1555-1562.Full Text via DOI: 10.1109/HPCC/SmartCity/DSS.2018.00255 Web of Science: 000468511200223
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2018Integration of Nanopore and Nanoelectrode for Single Entity Detection and Manipulation. 685A-685A.Web of Science: 000430563300419
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Letter
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Note
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2022Advanced nanoelectrochemistry implementation: from concept to application: general discussion. 354-373.Full Text via DOI: 10.1039/d2fd90004a
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Other Scholarly Work
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2020
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2020Nanoimpact Based Single-Entity Detection of Proteins using a Nanopore-Nanoelectrode Nanopipette. 472A-472A.Web of Science: 000513023203111
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2019Surface-Enhanced Raman Spectroscopy (SERS)-Active Nanopipette for Single Cell Intracellular pH Sensing. 146A-146A.Full Text via DOI: 10.1016/j.bpj.2018.11.812 Web of Science: 000460779800726
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2018Histology of NeoMTA Plus and Quick-Set2 in Contact with Pulp and Periradicular Tissues in a Canine Model.. 1389-1395.Full Text via DOI: 10.1016/j.joen.2018.05.001
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2018The Tsinghua-Lancet Commission on Healthy Cities in China: unlocking the power of cities for a healthy China.. 2140-2184.Full Text via DOI: 10.1016/s0140-6736(18)30486-0
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2017Extracellular Membrane Potential Measurement of Single Living Cells with Scanning Ion Conductance Microscopy. 587A-588A.Full Text via DOI: 10.1016/j.bpj.2016.11.3162 Web of Science: 000402375700919
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2016Theoretical Simulation and Experimental Investigation for the Identification and Analysis of Biphasic Surface Plasmon Resonance Data. 335A-335A.Full Text via DOI: 10.1016/j.bpj.2015.11.1804 Web of Science: 000375142200121
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2015Molecular Interaction between Escherichia Coli Topoisomerase I and pBAD/Thio Supercoiled Plasmid DNA. 398A-398A.Full Text via DOI: 10.1016/j.bpj.2014.11.2181 Web of Science: 000362849400415
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2014Quartz Nanopipettes for the Study of Protein-Protein Interaction. 620A-620A.Full Text via DOI: 10.1016/j.bpj.2013.11.3430 Web of Science: 000337000403478
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Review
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2010Recognition tunnelingFull Text via DOI: 10.1088/0957-4484/21/26/262001 Web of Science: 000278711100002
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Research
principal investigator on
- CAREER: Investigate 3D extracellular potential distribution at single cell level awarded by National Science Foundation 2015 - 2022
- NST ERC: Nanosystems Engineering Research Center for Directed Multiscale Assembly of Cellular Metamaterials with Nanoscale Precision: CELL-MET awarded by National Science Foundation 2018 - 2019
- Carbon nanotube based nanofluidic device for biological sensing awarded by National Science Foundation 2013 - 2018
co-principal investigator on
- Probing Molecular Dynamics Near Plasmonic Surfaces by Optical two-dimensional Coherent Spectroscopy awarded by National Science Foundation 2020 - 2023
- NST ERC: CELL-MET- Thrust 4 awarded by National Science Foundation 2019 - 2020
- NST ERC: Nanosystems Engineering Research Center for Directed Multiscale Assembly of Cellular Metamaterials with Nanoscale Precision: CELL-MET awarded by National Science Foundation 2018 - 2019
- NST ERC: Nanosystems Engineering Research Center for Directed Multiscale Assembly of Cellular Metamaterials with Nanoscale Precision: CELL-MET awarded by National Science Foundation 2017 - 2018
- Carbon nanotube based nanofluidic device for biological sensing awarded by National Science Foundation 2013 - 2018
subproject principal investigator on
investigator on
- Targeting the Caveolae-Dependent Mechanism of Calcifying Extracellular Vesicle Formation awarded by National Heart, Lung, & Blood Institute 2021 - 2026
- Science and Technology Center on Real-Time Functional Imaging (STROBE) awarded by National Science Foundation 2021 - 2026
- Thrust 4 : NSF ERC: Nanosystems Engineering Research Center for Directed Multiscale Assembly of Cellular Metamaterials with Nanoscale Precision: CELL-MET awarded by National Science Foundation 2021 - 2022
- NST ERC: CELL-MET- Thrust 4 awarded by National Science Foundation 2020 - 2021
- NST ERC: CELL-MET- Thrust 4 awarded by National Science Foundation 2019 - 2020
Contact
full name
- Jin He
Identifiers
ORCID iD
- https://orcid.org/0000-0002-2633-9809 (confirmed)
visualizations
publication subject areas
Citation index-derived subject areas the researcher has published in