Miksovska, Jaroslava
- Associate Professor, Chemistry and Biochemistry , College of Arts, Sciences & Education

Overview
overview
- Jaroslava Miksovska received her PhD in 1998 for time-resolved spectroscopic studies of electron and proton transfer in the photosynthetic reaction centers of Rhodobacter capsulatus under the supervision of Prof. Pierre Sebban at Centre de Genetique Moleculair, CNRS, Gif-sur-Yvette. After postdoctoral work in the group of Prof. Randy Larsen at University of Hawaii and then at University of South Florida, she took a position as Assistant Professor in the Chemistry Department, Marshall University. Since August 2007 she is an assistant professor in the Department of Chemistry and Biochemistry at Florida International University.
research interests
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Her research interests focus on the application of photoacoustic calorimetry and photothermal beam deflection to investigate variety of chemical and biochemical processes including conformational dynamics associated with ligand binding hemeproteins, conformational changes associated with Ca2+ binding to calcium binding proteins and, protein folding in reverse micelles.
Project 1: Downstream Regulatory Element Antagonist Modulator is a neuronal calcium sensor that regulates vital processes including potassium voltage channel kinetics, DNA transcription and calcium homeostasis. Our research effort focuses on understanding of how calcium/magnesium association to DREAM controls DREAM affinity to other bio-macromolecules such as DNA and presenilin and what is the role of small hydrophobic molecules in this process. Students working on this multi-disciplinary project will learn biochemical techniques (protein isolation and purification), biophysical techniques (fluorescence studies, ITC, CD), experimental data analysis approaches and basic computational techniques (homology modeling, protein docking).
Project 2: Hexa-coordinate globins neuroglobin and cytoglobin represent a new sub-family of vertebrate globins that are involved in tissue protection under condition of stress and hypoxia. Despite the fact that the physiological role of these proteins is relatively well understood, the molecular basis of their protective function reminds unclear. In our group we focus on understanding the protein structure/function/dynamic relationship of vertebrate hexa-coordinate proteins. Students working on this project will received training in protein purification an isolation, characterization heme proteins interaction with ligands using steady-state absorption spectroscopy, protein stability measurements, and basic of photothermal techniques.
Scholarly & Creative Works
selected publications
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Article
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2022Nanomolar affinity of EF-hands in neuronal calcium sensor 1 for bivalent cations Pb2+, Mn2+, and Hg2+Full Text via DOI: 10.1093/mtomcs/mfac039 Web of Science: 000822054200002
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2021Elucidation of specific binding sites and extraction of toxic Gen X from HSA employing cyclodextrinFull Text via DOI: 10.1016/j.jhazmat.2021.127765 Web of Science: 000752517300005
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2020Salt bridges govern the structural heterogeneity of heme protein interactions and porphyrin networks: microperoxidase-11Full Text via DOI: 10.1039/d0ra04956e Web of Science: 000571760600022
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2020Impact of azole drugs on energetics, kinetics, and ligand migration pathways of CO photo-dissociation in bacterial flavohemoglobinsFull Text via DOI: 10.1039/d0ra02529a Web of Science: 000534282700065
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2019Utility of fluorescent heme analogue ZnPPIX to monitor conformational heterogeneity in vertebrate hexa-coordinated globinsFull Text via DOI: 10.1039/c8mt00332g Web of Science: 000468785100002
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2019Fluorescent 5-Pyrimidine and 8-Purine Nucleosides Modified with an N-Unsubstituted 1,2,3-Triazol-4-yl MoietyFull Text via DOI: 10.1021/acs.joc.8b03135 Web of Science: 000461844000051
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2019The effects of solution additives and gas-phase modifiers on the molecular environment and conformational space of common heme proteinsFull Text via DOI: 10.1002/rcm.8347 Web of Science: 000459797600001
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20184,4 '-Dihydroxy-2,2 '-bipyridine complexes of Co(III), Cu(II) and Zn(II); structural and spectroscopic characterizationFull Text via DOI: 10.1016/j.poly.2018.04.039 Web of Science: 000437053900010
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2018Non-symbiotic hemoglobin conformational space dependence on the heme coordination using nESI-TIMS-TOF MSFull Text via DOI: 10.1016/j.ijms.2018.03.008 Web of Science: 000435178200006
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2018beta-Cyclodextrin Reverses Binding of Perfluorooctanoic Acid to Human Serum AlbuminFull Text via DOI: 10.1021/acs.chemrestox.8b00002 Web of Science: 000430436800009
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2017Role of Ionic Strength and pH in Modulating Thermodynamic Profiles Associated with CO Escape from Rice Nonsymbiotic Hemoglobin 1Full Text via DOI: 10.1021/acs.jpcb.6b06933 Web of Science: 000392554200002
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2016Fluorescently labeled circular DNA molecules for DNA topology and topoisomerasesFull Text via DOI: 10.1038/srep36006 Web of Science: 000386529400001
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2016Structures of the kinetically trapped i-motif DNA intermediatesFull Text via DOI: 10.1039/c6cp04418b Web of Science: 000385175000037
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2016Molecular insight of DREAM and presenilin 1 C-terminal fragment interactionsFull Text via DOI: 10.1002/1873-3468.12156 Web of Science: 000375008700009
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2016Characterization of the Photophysical, Thermodynamic, and Structural Properties of the Terbium(III)-DREAM ComplexFull Text via DOI: 10.1021/acs.biochem.6b00067 Web of Science: 000373247600019
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2015Amphiphilic Residues 29-44 of DREAM N-Termini Mediate Calmodulin:DREAM Complex FormationFull Text via DOI: 10.1021/acs.biochem.5b00251 Web of Science: 000358555300012
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2015Ca2+ and Mg2+ modulate conformational dynamics and stability of downstream regulatory element antagonist modulatorFull Text via DOI: 10.1002/pro.2646 Web of Science: 000353524700016
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2015Kinetic Intermediates of Holo- and Apo-Myoglobin Studied Using HDX-TIMS-MS and Molecular Dynamic SimulationsFull Text via DOI: 10.1007/s13361-014-1067-9 Web of Science: 000351351800005
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2015Site-specific dynamics of amyloid formation and fibrillar configuration of A beta(1-23) using an unnatural amino acidFull Text via DOI: 10.1039/c5cc00149h Web of Science: 000352630600027
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2014Flavin Adenine Dinucleotide Structural Motifs: From Solution to Gas PhaseFull Text via DOI: 10.1021/ac5023666 Web of Science: 000343639800033
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2014Application of ANS fluorescent probes to identify hydrophobic sites on the surface of DREAMFull Text via DOI: 10.1016/j.bbapap.2014.05.004 Web of Science: 000340339500005
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2014Emission Tuning of Fluorescent Kinase Inhibitors: Conjugation Length and Substituent EffectsFull Text via DOI: 10.1021/jo500520x Web of Science: 000337073900017
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2014Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam DeflectionFull Text via DOI: 10.3791/50969 Web of Science: 000348604100024
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2014Quantitative study of protein-protein interactions by quartz nanopipettesFull Text via DOI: 10.1039/c4nr02964j Web of Science: 000340520500046
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2013Reduction of the internal disulfide bond between Cys 38 and 83 switches the ligand migration pathway in cytoglobinFull Text via DOI: 10.1016/j.jinorgbio.2013.08.006 Web of Science: 000327171500004
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2012Photophysical Characterization of a Benzo-Fused Analogue of Brooker's Merocyanine: Solvent Polarity and pH EffectsFull Text via DOI: 10.1021/jp3106869 Web of Science: 000312681800009
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2012Conformational Dynamics in Human Neuroglobin: Effect of His64, Val68, and Cys120 on Ligand MigrationFull Text via DOI: 10.1021/bi301016u Web of Science: 000312430100004
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2012Graphene Oxide as a Quencher for Fluorescent Assay of Amino Acids, Peptides, and ProteinsFull Text via DOI: 10.1021/am302704a Web of Science: 000313149800088
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2012Emission Switching of 4,6-Diphenylpyrimidones: Solvent and Solid State EffectsFull Text via DOI: 10.1021/jp3036956 Web of Science: 000308119800002
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2012Isolation, characterization and antioxidative activity of C-phycocyanin from Limnothrix sp strain 37-2-1Full Text via DOI: 10.1016/j.jbiotec.2012.02.004 Web of Science: 000302772000003
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2011Functionalization of rho-ZMOF with photosensitizers for singlet oxygen generationFull Text via DOI: 10.1016/j.jphotochem.2011.04.029 Web of Science: 000292488400011
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2010Probing the Role of the Internal Disulfide Bond in Regulating Conformational Dynamics in NeuroglobinFull Text via DOI: 10.1016/j.bpj.2010.04.033 Web of Science: 000280182300002
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2009Photothermal studies of CO photodissociation from peroxidases from horseradish and soybeanFull Text via DOI: 10.1016/j.bbapap.2009.06.021 Web of Science: 000270763900002
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2009Thermodynamic profile for urea photo-release from a N-(2-nitrobenzyl) caged urea compoundFull Text via DOI: 10.1039/b900593e Web of Science: 000268481000009
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2008Conformational Dynamics Associated with Photodissociation of CO from Dehaloperoxidase Studied Using Photoacoustic CalorimetryFull Text via DOI: 10.1021/bi8012033 Web of Science: 000260507100015
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2005Effects of turn stability on the kinetics of refolding of a hairpin in a β-sheetFull Text via DOI: 10.1021/ja0543191
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2004Ligand binding subsequent to NO photolyses of partially unfolded cytochrome cFull Text via DOI: 10.1002/jccs.200400167
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2003Volume and enthalpy profiles of CO rebinding to horse heart myoglobinFull Text via DOI: 10.1007/s00775-003-0457-4
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2003Photothermal studies of pH induced unfolding of apomyoglobinFull Text via DOI: 10.1023/A:1025398325578
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2003Structure-function relationships in metalloproteinsFull Text via DOI: 10.1016/S0076-6879(03)60117-5
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Book Chapter
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2011Time Resolved Thermodynamics Associated with Diatomic Ligand Dissociation from Globins. 301-318.Web of Science: 000378839400012
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1995Cyanobacterial Photosystem II Stability. Effect of N-Trimethylglycine. 2329-2332.Full Text via DOI: 10.1007/978-94-009-0173-5_549
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1995Electrostatic Interaction Between L212Glu and QA − in Bacterial Reaction Centers. 467-470.Full Text via DOI: 10.1007/978-94-009-0173-5_107
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Conference
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2021Role of the Trp169 Residue in Neuronal Calcium Sensor Dream. 296A-296A.Web of Science: 000629601401675
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2019The Effect of Lithium Binding on Secondary and Tertiary Structure, Hydrophobicity, Thermodynamics, and Interactions with Interacting Partners of Dream. 48A-48A.Full Text via DOI: 10.1016/j.bpj.2018.11.305 Web of Science: 000460779800243
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2018
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2018
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2018
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2017Lead Tightly Associates to Neuronal Calcium Sensor (NCS) Protein Dream and Promotes Structural Changes Analogous to Calcium Bound Dream. 398A-398A.Full Text via DOI: 10.1016/j.bpj.2016.11.2160 Web of Science: 000402375600977
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2013Conformational dynamics associated with the ligand migration in hexa-coordinate globins. S161-S161.Web of Science: 000330215300483
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2013Gas-phase, conformational dynamics hexa-coordinated globin proteins. S70-S70.Web of Science: 000330215300133
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2012
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2011
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2010
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2009Unraveling structural dynamics in heme proteinsWeb of Science: 000207862000708
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Other Scholarly Work
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2022Distinct mechanism of interactions between NCS1 and Tb3+/Eu3+. 453A-453A.Web of Science: 000759523003016
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2022Mechanism of DREAM oligomerization kinetic study. 336A-336A.Web of Science: 000759523002183
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2021Conformational Structural Alteration of NCS1 Protein Through Pb2+ and Mn2+ Association. 296A-296A.Web of Science: 000629601401677
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2020Fluorescent Analogue of Hexa-coordinate Globins to Monitor Accessibility of the Heme Pocket. 355A-356A.Web of Science: 000513023202275
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2020Impact of the Internal Disulfide Bond on the Ligand Migration in Globin X. 514A-514A.Web of Science: 000513023203315
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2020The Molecular Mechanism of Ca2+ Triggered DREAM Dimerization. 356A-356A.Web of Science: 000513023202278
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2019Mutation of Residues in CD Loop and Distal Pocket Impact Protein Stability of Human Neuroglobin. 471A-471A.Full Text via DOI: 10.1016/j.bpj.2018.11.2545 Web of Science: 000460779802366
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2019Neuronal Calcium Sensor Dream Interactions with Insulinotropic Agent Repaglinide. 471A-471A.Full Text via DOI: 10.1016/j.bpj.2018.11.2543 Web of Science: 000460779802364
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2018Determination of Effector Binding Affinities Using Photoacoustic Calorimetry. 416A-416A.Full Text via DOI: 10.1016/j.bpj.2017.11.2306 Web of Science: 000430450000570
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2018Distinct Mechanism of Oxygen and Carbon Monoxide Interactions with HEME Protein. 419A-419A.Web of Science: 000430450000588
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2018
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2018Reactivity of Hexacoordinated HEME Proteins on Ultrafast Timescales. 419A-419A.Web of Science: 000430450000587
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2017Determination of Ligand Migration Pathways in Human Cytoglobin. 494A-494A.Full Text via DOI: 10.1016/j.bpj.2016.11.2674 Web of Science: 000402375700460
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2017Effects of Azole Drugs on Conformational Dynamics and Energetics Associated with Diatomic Ligand Photorelease and Migration within Bacterial Flavohemoglobins. 495A-495A.Full Text via DOI: 10.1016/j.bpj.2016.11.2679 Web of Science: 000402375700465
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2016Characterization of the Role of Individual EF-Hands in Dream in Modulating Conformational Dynamics, Oligomeric States, and Interaction with DNA. 489A-489A.Full Text via DOI: 10.1016/j.bpj.2015.11.2615 Web of Science: 000375142700379
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2016Determination of Thermodynamic and Kinetic Parameters for Co Migration within Bacterial Flavohemoglobins of Ralstonia Eutropha and Staphylococcus Aureus. 223A-223A.Full Text via DOI: 10.1016/j.bpj.2015.11.1236 Web of Science: 000375141600089
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2016Dynamic Network Analysis of Dream Protein Dimer Interface. 382A-382A.Full Text via DOI: 10.1016/j.bpj.2015.11.2062 Web of Science: 000375142200351
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2016Role or Aromatic Residues in Dynamic Networks in DREAM/KChIP3. 513A-513A.Full Text via DOI: 10.1016/j.bpj.2015.11.2744 Web of Science: 000375142700500
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2015Characterization of the Photophysical, Thermodynamic and Structural Properties of the Terbium(III)-KChIP3 Complex. 218A-218A.Full Text via DOI: 10.1016/j.bpj.2014.11.1203 Web of Science: 000362849100302
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2015Identify the Binding Interface between Calsenilin and Presenilin 1 C-Terminal Fragment. 108A-108A.Full Text via DOI: 10.1016/j.bpj.2014.11.614 Web of Science: 000359471700545
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2015Role of Ionic Strength and the Bohr Effect in Modulating Thermodynamic Profiles Associated with Co Escape in Rice Non-Symbiotic Hemoglobin 1. 57A-58A.Full Text via DOI: 10.1016/j.bpj.2014.11.348 Web of Science: 000359471700289
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2015The N-Terminal Extension of KChIP3 is Responsible for KChIP3-Calmodulin Complex Formation. 279A-279A.Full Text via DOI: 10.1016/j.bpj.2014.11.1526 Web of Science: 000362849100611
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2014DNA i-MOTIF Probed by Photoacoustic Calorimetry. 64A-64A.Full Text via DOI: 10.1016/j.bpj.2013.11.434 Web of Science: 000337000400330
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2014Molecular Mechanism of Dream - Presenilin-1 Interactions. 528A-528A.Full Text via DOI: 10.1016/j.bpj.2013.11.2947 Web of Science: 000337000403018
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2014Study of the Dynamic and Thermodynamic Calcium Induced Transition in the Downstream Regulatory Element Antagonist Modulator (DREAM) using Photothermal Beam Deflection (PBD). 659A-659A.Full Text via DOI: 10.1016/j.bpj.2013.11.3650 Web of Science: 000337000403679
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2013Cytoglobin Interactions with Hydrophobic Probe 1,8-ANS. 563A-563A.Full Text via DOI: 10.1016/j.bpj.2012.11.3119 Web of Science: 000316074305373
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2013Modulation of the KV4.3-KchIP3 Interactions by Ca2+ and NS5806. 102A-102A.Full Text via DOI: 10.1016/j.bpj.2012.11.602 Web of Science: 000316074301029
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2013Spectroscopic Study Ca2+ Induced Changes in the Structure, Dynamics and Stability of Dream Protein and its Mechanism of DNA Interaction. 102A-102A.Full Text via DOI: 10.1016/j.bpj.2012.11.601 Web of Science: 000316074301028
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2013Time-Resolved Thermodynamics and Transient Kinetics for Oxygen Photorelease from Hemoglobin: Effector Complexes. 559A-559A.Full Text via DOI: 10.1016/j.bpj.2012.11.3100 Web of Science: 000316074305354
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2012Ca2+ and Mg2+ Induced Conformational Changes in Downstream Regulatory Antagonist Modulator (DREAM) Measured by Extrinsic Hydrophobic Probes. 507A-507A.Full Text via DOI: 10.1016/j.bpj.2011.11.2780 Web of Science: 000321561203456
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2012Impact of the Internal Disulfide Bond on Structural Dynamics in Vertebrates Hexacoordinate Hemeoglobins. 465A-465A.Full Text via DOI: 10.1016/j.bpj.2011.11.2552 Web of Science: 000321561203237
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2012Mg2+/Ca2+ Induced Changes in Structure, Dynamics and Stability of Dream Protein. 507A-508A.Full Text via DOI: 10.1016/j.bpj.2011.11.2781 Web of Science: 000321561203457
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2010Characterization of Conformational Transitions in Dream Protein using Fluorescence Spectroscopy. 675A-675A.Full Text via DOI: 10.1016/j.bpj.2009.12.3707 Web of Science: 000208762006394
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2010Evaluation of Photophysical Properties of Methylene Blue Incorporated within ZMOF Framework. 404A-404A.Full Text via DOI: 10.1016/j.bpj.2009.12.2176 Web of Science: 000208762004011
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2010Structural Dynamics in Chloroperoxidase: A Photoacoustic Study. 643A-643A.Full Text via DOI: 10.1016/j.bpj.2009.12.3523 Web of Science: 000208762006221
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2010The Role of the Internal Disulfide Bond in the Conformational Dynamics of Neuroglobin. 639A-639A.Full Text via DOI: 10.1016/j.bpj.2009.12.3503 Web of Science: 000208762006201
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2009Characterization Of Ca2+ Photo-release From DM-nitrophen Using Photothermal Beam Deflection. 118A-118A.Full Text via DOI: 10.1016/j.bpj.2008.12.519 Web of Science: 000426275900599
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2009Conformatinal Dynamics Of Cytochrome c Encapsulated In AOT Reverse Micelles. 438A-438A.Full Text via DOI: 10.1016/j.bpj.2008.12.2245 Web of Science: 000426354000335
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2009Dynamics And Energetics Associated With Ligand Photodissociation From Co Bound Chloroperoxidase. 437A-437A.Full Text via DOI: 10.1016/j.bpj.2008.12.2242 Web of Science: 000426354000332
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Preprint
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2022Globin X: A Highly Stable Intrinsically Hexa-Coordinate GlobinFull Text via DOI: 10.2139/ssrn.4150472
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Review
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2011Time resolved thermodynamics associated with ligand photorelease in heme peroxidases and globins: Open access channels versus gated ligand release. 1065-1076.Full Text via DOI: 10.1016/j.bbapap.2011.01.009 Web of Science: 000292350200013
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2007Time resolved thermodynamics of ligand binding to heme proteins. 1101-1127.Full Text via DOI: 10.1016/j.ccr.2006.08.018
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Works By Students
chaired theses and dissertations
- Butcher, David S, Thermodynamics and Kinetics of Ligand Photodissociation in Heme Proteins and Formation of DNA i-Motif 2017
- Pham, Khoa Ngoc, Conformational Dynamics and Stability Associated with Magnesium or Calcium Binding to DREAM in the Regulation of Interactions between DREAM and DNA or Presenilins 2016
- Gonzalez, Walter G, Protein-Ligand Interactions and Allosteric Regulation of Activity in DREAM Protein 2016
- Astudillo, Luisana, Conformational Dynamics Associated with Ligand Binding to Vertebrate Hexa-coordinate Hemoglobins 2014
- Dhulipala, Gangadhar, Conformational dynamics associated with calcium binding to calcium transducers 2010
advised honors theses
- Betancourt, Jovany Jordan, Analysis of Structural Changes Caused by the Association of Small Molecules with Globular Proteins 2019
Research
principal investigator on
- Conformational Dynamics Associated with Ca2+ Binding to DREAM Protein awarded by National Science Foundation 2010 - 2014
- Conformational Dynamics in Vertebrate Hexacoordiante Hemoglobins awarded by Florida Department of Health 2010 - 2013
- Thermodynamics Profiles for Calcium Signaling. awarded by American Heart Association 2007 - 2009
co-principal investigator on
Contact
full name
- Jaroslava Miksovska
Identifiers
ORCID iD
- https://orcid.org/0000-0002-0385-9457 (confirmed)
visualizations
publication subject areas
Citation index-derived subject areas the researcher has published in