Rosen, Barry
- Distinguished University Professor, Cellular Biology and Pharmacology , Herbert Wertheim College of Medicine

Overview
overview
- Barry P. Rosen was Associate Dean from 2009-2016. For 22 years he was Chair and Distinguished Professor of Biochemistry and Molecular Biology at Wayne State University School of Medicine. He has more than 350 publications and numerous awards, including Basil O'Connor Award from the March of Dimes, Maryland Distinguished Young Scientist Award, Josiah Macy, Jr. Faculty Scholar Award and FIU Top Scholar Award. Rosen has been continuously funded by NIH and NSF since 1972. He held a prestigious NIH MERIT Award, renewed as an R01 and most recently as a MIRA Award until 2025, putting it within the top 100 longest continuously funded NIH grants. He has been on many grant panels and editorial boards. He was President of the Association of Medical and Graduate Departments of Biochemistry and is a fellow of the American Academy for Microbiology (ASM) and the American Association for the Advancement of Science (AAAS).
research interests
- For four decades Dr. Rosen has investigated the mechanisms of transport and detoxification of transition metals, heavy metals and metalloids in bacteria, yeast, protozoans, mammals and plants. He identified the pathways of arsenic uptake, efflux, biotransformation and regulation in organisms from E. coli to humans. He identified most of the known arsenic detoxification genes and characterized their gene products at the biochemical and structural level. He solved the crystal structure of the ArsA As(III)-translocating ATPase, the ArsR repressor orthologue CadC, the ArsC and LmAcr2 arsenate reductases, the ArsH NADPH-FMN oxidoreductase, the ArsD As(III) metallochaperone, the ArsM As(III)-SAM methyltransferases from Cyanidioschyzon merolae and Chlamydomonas reinhardtii and most recently the ArsI C-As lyase. He identified and named the ArsR family of metalloregulatory proteins. He made the seminal discovery that aquaglyceroporin channels, from E. coli GlpF to human AQP9, are the transporters that nearly every cell uses to take up As(III). He is currently elucidating the enzymes and transporters of the arsenic biomethylation and organoarsenical redox cycles.
Scholarly & Creative Works
selected publications
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Article
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2021Comamonas testosteroni antA encodes an antimonite-translocating P-type ATPase. SCIENCE OF THE TOTAL ENVIRONMENT. 754.Full Text via DOI: 10.1016/j.scitotenv.2020.142393 Web of Science: 000593907600012
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2020The Pseudomonas putida NfnB nitroreductase confers resistance to roxarsone. SCIENCE OF THE TOTAL ENVIRONMENT. 748.Full Text via DOI: 10.1016/j.scitotenv.2020.141339 Web of Science: 000581049800047
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2020Semisynthesis of the Organoarsenical Antibiotic Arsinothricin. JOURNAL OF NATURAL PRODUCTS. 83:2809-2813.Full Text via DOI: 10.1021/acs.jnatprod.0c00522 Web of Science: 000575848000032
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2020Organoarsenicals inhibit bacterial peptidoglycan biosynthesis by targeting the essential enzyme MurA. CHEMOSPHERE. 254.Full Text via DOI: 10.1016/j.chemosphere.2020.126911 Web of Science: 000538150400126
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2020The Great Oxidation Event expanded the genetic repertoire of arsenic metabolism and cycling. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 117:10414-10421.Full Text via DOI: 10.1073/pnas.2001063117 Web of Science: 000532837500042
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2020Comparative Cytotoxicity of Inorganic Arsenite and Methylarsenite in Human Brain Cells. ACS CHEMICAL NEUROSCIENCE. 11:743-751.Full Text via DOI: 10.1021/acschemneuro.9b00653 Web of Science: 000518702400007
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2019Reduction of Organoarsenical Herbicides and Antimicrobial Growth Promoters by the Legume Symbiont Sinorhizobium meliloti. ENVIRONMENTAL SCIENCE & TECHNOLOGY. 53:13648-13656.Full Text via DOI: 10.1021/acs.est.9b04026 Web of Science: 000500838900011
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2019Genomewide Analysis of Mode of Action of the S-Adenosylmethionine Analogue Sinefungin in Leishmania infantum. MSYSTEMS. 4.Full Text via DOI: 10.1128/mSystems.00416-19 Web of Science: 000500493300002
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2019Structures of two ArsR As(III)-responsive transcriptional repressors: Implications for the mechanism of derepression. JOURNAL OF STRUCTURAL BIOLOGY. 207:209-217.Full Text via DOI: 10.1016/j.jsb.2019.05.009 Web of Science: 000477692100010
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2019Role of ArsEFG in Roxarsone and Nitarsone Detoxification and Resistance. ENVIRONMENTAL SCIENCE & TECHNOLOGY. 53:6182-6191.Full Text via DOI: 10.1021/acs.est.9b01187 Web of Science: 000470793900008
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2019Arsinothricin, an arsenic-containing non-proteinogenic amino acid analog of glutamate, is a broad-spectrum antibiotic. COMMUNICATIONS BIOLOGY. 2.Full Text via DOI: 10.1038/s42003-019-0365-y Web of Science: 000465423400001
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2019The antibiotic action of methylarsenite is an emergent property of microbial communities. MOLECULAR MICROBIOLOGY. 111.Full Text via DOI: 10.1111/mmi.14169 Web of Science: 000458286600012
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2019Identification of Steps in the Pathway of Arsenosugar Biosynthesis. ENVIRONMENTAL SCIENCE & TECHNOLOGY. 53.Full Text via DOI: 10.1021/acs.est.8b04389 Web of Science: 000456349900009
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2018Arsenic-hypertolerant and arsenic-reducing bacteria isolated from wells in Tucuman, Argentina. CANADIAN JOURNAL OF MICROBIOLOGY. 64.Full Text via DOI: 10.1139/cjm-2017-0535 Web of Science: 000448727200011
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2018Directed Evolution of Saccharomyces cerevisiae for Increased Selenium Accumulation. MICROORGANISMS. 6.Full Text via DOI: 10.3390/microorganisms6030081 Web of Science: 000446334200026
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2018The Structure of an As(III) S-Adenosylmethionine Methyltransferase with 3-Coordinately Bound As(III) Depicts the First Step in Catalysis. BIOCHEMISTRY. 57.Full Text via DOI: 10.1021/acs.biochem.8b00457 Web of Science: 000439397900006
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2018Reorientation of the Methyl Group in MAs(III) is the Rate-Limiting Step in the ArsM As(III) S-Adenosylmethionine Methyltransferase Reaction. ACS OMEGA. 3.Full Text via DOI: 10.1021/acsomega.8b00197 Web of Science: 000427939400068
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2018Arsenic methylation by a novel ArsM As(III) S-adenosylmethionine methyltransferase that requires only two conserved cysteine residues. MOLECULAR MICROBIOLOGY. 107:265-276.
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2017A novel MAs(III)-selective ArsR transcriptional repressor. MOLECULAR MICROBIOLOGY. 106:469-478.
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2017Arsenic methylation by a genetically engineered Rhizobium-legume symbiont. PLANT AND SOIL. 416:259-269.
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2017Biochemical Characterization of Arsl: A Novel C-As Lyase for Degradation of Environmental Organoarsenicals. ENVIRONMENTAL SCIENCE & TECHNOLOGY. 51:11115-11125.
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2017Conserved cysteine residues determine substrate specificity in a novel As(III) S-adenosylmethionine methyltransferase from Aspergillus fumigatus. MOLECULAR MICROBIOLOGY. 104:250-259.
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2017Nonsynonymous Polymorphisms in the Human AS3MT Arsenic Methylation Gene: Implications for Arsenic Toxicity. CHEMICAL RESEARCH IN TOXICOLOGY. 30:1481-1491.
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2017Recurrent horizontal transfer of arsenite methyltransferase genes facilitated adaptation of life to arsenic. SCIENTIFIC REPORTS. 7.
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2017Structural studies of the ArsD arsenic metallochaperone using molecular dynamics. JOURNAL OF COMPUTATIONAL METHODS IN SCIENCES AND ENGINEERING. 17.Full Text via DOI: 10.3233/JCM-170718 Web of Science: 000400919200003
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2016Arsenic Directly Binds to and Activates the Yeast AP-1-Like Transcription Factor Yap8. MOLECULAR AND CELLULAR BIOLOGY. 36:913-922.
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2016Arsenic Methylation in Arabidopsis thaliana Expressing an Algal Arsenite Methyltransferase Gene Increases Arsenic Phytotoxicity. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 64:2674-2681.
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2016Characterization of the extremely arsenic-resistant Brevibacterium linens strain AE038-8 isolated from contaminated groundwater in Tucuman, Argentina. INTERNATIONAL BIODETERIORATION & BIODEGRADATION. 107:147-153.Full Text via DOI: 10.1016/j.ibiod.2015.11.022 Web of Science: 000369206700020
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2016Efficient Arsenic Methylation and Volatilization Mediated by a Novel Bacterium from an Arsenic-Contaminated Paddy Soil. ENVIRONMENTAL SCIENCE & TECHNOLOGY. 50:6389-6396.
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2016Inositol transporters AtINT2 and AtINT4 regulate arsenic accumulation in Arabidopsis seeds. NATURE PLANTS. 2.
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2016Organoarsenical Biotransformations by Shewanella putrefaciens. ENVIRONMENTAL SCIENCE & TECHNOLOGY. 50:7956-7963.
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2016Structure of the Arsl C-As Lyase: Insights into the Mechanism of Degradation of Organoarsenical Herbicides and Growth Promoters. JOURNAL OF MOLECULAR BIOLOGY. 428:2462-2473.
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2016Synergistic interaction of glyceraldehydes-3-phosphate dehydrogenase and ArsJ, a novel organoarsenical efflux permease, confers arsenate resistance. MOLECULAR MICROBIOLOGY. 100:945-953.
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2015A disulfide-bond cascade mechanism for arsenic(III) S-adenosylmethionine methyltransferase. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY. 71:505-515.
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2015ArsH is an organoarsenical oxidase that confers resistance to trivalent forms of the herbicide monosodium methylarsenate and the poultry growth promoter roxarsone. MOLECULAR MICROBIOLOGY. 96:1042-1052.
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2015Arsenic Methylation and Volatilization by Arsenite S-Adenosylmethionine Methyltransferase in Pseudomonas alcaligenes NBRC14159. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 81:2852-2860.
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2015As(III) S-Adenosylmethionine Methyltransferases and Other Arsenic Binding Proteins. GEOMICROBIOLOGY JOURNAL. 32:570-576.
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2015Genetically Engineering Bacillus subtilis with a Heat-Resistant Arsenite Methyltransferase for Bioremediation of Arsenic-Contaminated Organic Waste. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 81:6718-6724.
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2015High-throughput screening-compatible assays of As(III) S-adenosylmethionine methyltransferase activity. ANALYTICAL BIOCHEMISTRY. 480:67-73.
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2015Identification of Small Molecule Inhibitors of Human As(III) S-Adenosylmethionine Methyltransferase (AS3MT). CHEMICAL RESEARCH IN TOXICOLOGY. 28:2419-2425.
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2014A C center dot As lyase for degradation of environmental organoarsenical herbicides and animal husbandry growth promoters. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 111:7701-7706.
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2014Biosensor for Organoarsenical Herbicides and Growth Promoters. ENVIRONMENTAL SCIENCE & TECHNOLOGY. 48:1141-1147.
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2014Crystallization and preliminary X-ray crystallographic studies of CrArsM, an arsenic(III) S-adenosylmethionine methyltransferase from Chlamydomonas reinhardtii. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS. 70:1385-1388.
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2014Crystallization and preliminary X-ray crystallographic studies of the ArsI C-As lyase from Thermomonospora curvata. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS. 70:761-764.
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2014Mutations in the ArsA ATPase that restore interaction with the ArsD metallochaperone. BIOMETALS. 27:1263-1275.
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2014Structure of Escherichia coli Grx2 in complex with glutathione: a dual-function hybrid of glutaredoxin and glutathione S-transferase. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY. 70:1907-1913.
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2014Volatilization of Arsenic from Polluted Soil by Pseudomonas putida Engineered for Expression of the arsM Arsenic(III) S-Adenosine Methyltransferase Gene. ENVIRONMENTAL SCIENCE & TECHNOLOGY. 48:10337-10344.
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2013Determinants of the tumor suppressor INPP4B protein and lipid phosphatase activities. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 440:277-282.
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2013Engineering the Soil Bacterium Pseudomonas putida for Arsenic Methylation. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 79:4493-4495.
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2012A 3D localized surface plasmon resonance biosensor for the study of trivalent arsenic binding to the ArsA ATPase. BIOSENSORS & BIOELECTRONICS. 38:19-26.
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2012A Novel Biosensor Selective for Organoarsenicals. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 78:7145-7147.
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2012Efflux Permease CgAcr3-1 of Corynebacterium glutamicum Is an Arsenite-specific Antiporter. JOURNAL OF BIOLOGICAL CHEMISTRY. 287:723-735.
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2012Identification of Catalytic Residues in the As(III) S-Adenosylmethionine Methyltransferase. BIOCHEMISTRY. 51:944-951.
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2012Structure of an As(III) S-Adenosylmethionine Methyltransferase: insights into the Mechanism of Arsenic Biotransformation. BIOCHEMISTRY. 51:5476-5485.
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2011Biotransformation and Volatilization of Arsenic by Three Photosynthetic Cyanobacteria. PLANT PHYSIOLOGY. 156:1631-1638.
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2011Demethylation of methylarsonic acid by a microbial community. ENVIRONMENTAL MICROBIOLOGY. 13:1205-1215.
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2011Genetic mapping of the interface between the ArsD metallochaperone and the ArsA ATPase. MOLECULAR MICROBIOLOGY. 79:872-881.
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2011Purification, crystallization and preliminary X-ray diffraction studies of the arsenic repressor ArsR from Corynebacterium glutamicum. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS. 67:1616-1618.
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2011Resonance assignments and secondary structure prediction of the As(III) metallochaperone ArsD in solution. BIOMOLECULAR NMR ASSIGNMENTS. 5:109-112.
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2010Adventitious Arsenate Reductase Activity of the Catalytic Domain of the Human Cdc25B and Cdc25C Phosphatases. BIOCHEMISTRY. 49:802-809.
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2010Biochemical characterization of a novel ArsA ATPase complex from Alkaliphilus metalliredigens QYMF. FEBS LETTERS. 584:3089-3094.
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2010Crystallization and preliminary X-ray diffraction analysis of rat autotaxin. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS. 66:1050-1052.
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2010Jen1p: A High Affinity Selenite Transporter in Yeast. MOLECULAR BIOLOGY OF THE CELL. 21:3934-3941.
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2010Role of Signature Lysines in the Deviant Walker A Motifs of the ArsA ATPase. BIOCHEMISTRY. 49:356-364.
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2010The 1.4 angstrom Crystal Structure of the ArsD Arsenic Metallochaperone Provides Insights into Its interaction with the ArsA ATPase. BIOCHEMISTRY. 49:5206-5212.
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2010Trivalent arsenicals and glucose use different translocation pathways in mammalian GLUT1. METALLOMICS. 2:211-219.
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2009Biotransformation of arsenic by a Yellowstone thermoacidophilic eukaryotic alga. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 106.Full Text via DOI: 10.1073/pnas.0900238106 Web of Science: 000264790600048
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2009Properties of Arsenite Efflux Permeases (Acr3) from Alkaliphilus metalliredigens and Corynebacterium glutamicum. JOURNAL OF BIOLOGICAL CHEMISTRY. 284:19887-19895.
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2009Reduced arsenic clearance and increased toxicity in aquaglyceroporin-9-null mice. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 106:15956-15960.
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2009Role of Bound Zn(II) in the CadC Cd(II)/Pb(II)/Zn(II)-responsive Repressor. JOURNAL OF BIOLOGICAL CHEMISTRY. 284:14958-14965.
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2009Transport Mechanisms of Resistance to Drugs and Toxic Metals. ANTIMICROBIAL DRUG RESISTANCE, VOL 1: MECHANISMS OF DRUG RESISTANCE. 111-120.Full Text via DOI: 10.1007/978-1-59745-180-2_10 Web of Science: 000268730400010
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Book
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Book Chapter
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2014Earth Abides Arsenic Biotransformations. ANNUAL REVIEW OF EARTH AND PLANETARY SCIENCES, VOL 42. 443-+.Full Text via DOI: 10.1146/annurev-earth-060313-054942 PMID: 26778863 Web of Science: 000348436700020
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2012Pathways of Arsenic Uptake and Efflux. METAL TRANSPORTERS. 325-358.Full Text via DOI: 10.1016/B978-0-12-394390-3.00012-4 PMID: 23046656 Web of Science: 000314729900013
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Conference
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Editorial Material
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Letter
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2014Response to the Letter to the Editor Regarding Our Article (Dheeman et al., 2014). CHEMICAL RESEARCH IN TOXICOLOGY. 2012-2012.
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Meeting Abstract
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2019Humic acid coated iron oxide magnetic nanoparticles (HA-MNP) as potential photosensitizer for the oxidation and subsequent adsorption of arsenite. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.Web of Science: 000478861200565
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2018ArsI, a C-As lyase for degradation of environmental organoarsenicals. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.Web of Science: 000447609106239
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2018Novel biosensor for organoarsenical herbicides and antimicrobial growth promoters. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.Web of Science: 000447609100050
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2015Arsenic directly binds and activates the yeast AP-1-like transcription factor Yap8. YEAST. S223-S224.Web of Science: 000361466200381
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2014Enzymes of arsenic biotransformation. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY. S178-S178.Web of Science: 000332835300120
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Proceedings Paper
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2012Pathways of arsenic biotransformations: The arsenic methylation cycle. UNDERSTANDING THE GEOLOGICAL AND MEDICAL INTERFACE OF ARSENIC, AS 2012. 185-188.Web of Science: 000393139700065
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2012The arsenic stress defense mechanism of Corynebaterium glutamicum revealed. UNDERSTANDING THE GEOLOGICAL AND MEDICAL INTERFACE OF ARSENIC, AS 2012. 209-210.Web of Science: 000393139700073
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Review
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2022The East Africa Consortium for human papillomavirus and cervical cancer in women living with HIV/AIDS. 1202-1211.Full Text via DOI: 10.1080/07853890.2022.2067897
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2020The Arsenic Methylation Cycle: How Microbial Communities Adapted Methylarsenicals for Use as Weapons in the Continuing War for Dominance. FRONTIERS IN ENVIRONMENTAL SCIENCE.Full Text via DOI: 10.3389/fenvs.2020.00043 Web of Science: 000531624300001
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2019Organoarsenical compounds: Occurrence, toxicology and biotransformation. CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY.Full Text via DOI: 10.1080/10643389.2019.1619375 Web of Science: 000473043900001
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2019Pathways of arsenic uptake and efflux. ENVIRONMENT INTERNATIONAL. 585-597.Full Text via DOI: 10.1016/j.envint.2019.02.058 Web of Science: 000462597500067
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2018Thiolation in arsenic metabolism: a chemical perspective. METALLOMICS.Full Text via DOI: 10.1039/c8mt00231b Web of Science: 000448343400002
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2017Linking Genes to Microbial Biogeochemical Cycling: Lessons from Arsenic. ENVIRONMENTAL SCIENCE & TECHNOLOGY. 7326-7339.
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2016The organoarsenical biocycle and the primordial antibiotic methylarsenite. METALLOMICS. 1047-1055.
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2014Aquaglyceroporins: Generalized metalloid channels. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS. 1583-1591.
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Works By Students
chaired theses and dissertations
- Li, Jiaojiao, Arsenic Biotransformations in Microbes and Humans, and Catalytic Properties of Human AS3MT Variants 2017
- Pillai, Jitesh Kannan, Mechanisms of Arsenic Detoxification and Resistance 2014
Research
principal investigator on
- Mechanisms of arsenic transport and biotransformations awarded by General Medical Sciences 2020 - 2025
- Mechanisms of Arsenical Biotransformations and Transport awarded by General Medical Sciences 2016 - 2022
- The Human Arsenic Methylation Pathway awarded by Environmental Health Sciences 2014 - 2021
- Mechanisms of arsenical transport awarded by General Medical Sciences 2010 - 2016
- Development of high-throughput assays to identify drugs to prevent arsenic carcinogenesis awarded by Florida Department of Health 2013 - 2015
- Metal Binding Domains in Metalloregulatory Proteins awarded by Allergy and Infectious Diseases 2009 - 2011
- Mechanisms of Arsenical Transport. awarded by General Medical Sciences 2009 - 2010
Videos
videos
Contact
full name
- Barry Rosen
Identifiers
ORCID iD
- https://orcid.org/0000-0002-5230-4271 (confirmed)
visualizations
Recent publications and grants in Scholars@FIU
publication subject areas
- Agriculture
- Astronomy & Astrophysics
- Biochemistry & Molecular Biology
- Biophysics
- Biotechnology & Applied Microbiology
- Cell Biology
- Chemistry
- Crystallography
- Electrochemistry
- Engineering
- Environmental Sciences & Ecology
- Food Science & Technology
- Geology
- Life Sciences & Biomedicine - Other Topics
- Microbiology
- Mycology
- Pharmacology & Pharmacy
- Plant Sciences
- Science & Technology - Other Topics
- Spectroscopy
- Toxicology