Wang, Xiaotang
- Associate Professor, Chemistry and Biochemistry , College of Arts, Sciences & Education
Contact Info
Overview
research interests
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Structural and functional characterization of heme proteins containing thiolate axial ligand
Design and engineering of metalloproteins for asymmetric catalysis
Enzymatic synthesis of chiral synthons
NMR spectroscopic study of the active site structure of paramagnetic metalloproteins
The role of metal ions in cancer and neurodegenerative diseases
Scholarly & Creative Works
selected publications
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Article
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2020DNA and RNA editing without sequence limitation using the flap endonuclease 1 guided by hairpin DNA probes. NUCLEIC ACIDS RESEARCH. 48.Full Text via DOI: 10.1093/nar/gkaa843 Web of Science: 000606018600003
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2020Improving the thermostability of a GH97 dextran glucosidase by rational design. BIOTECHNOLOGY LETTERS.Full Text via DOI: 10.1007/s10529-020-02928-8 Web of Science: 000537407400004
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2020An unusual GH1 beta-glucosidase from marine sediment with beta-galactosidase and transglycosidation activities for superior galacto-oligosaccharide synthesis. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 104:4927-4943.Full Text via DOI: 10.1007/s00253-020-10578-z Web of Science: 000525476800001
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2020An oroxylin A-loaded aggregation-induced emission active polymeric system greatly increased the antitumor efficacy against squamous cell carcinoma. JOURNAL OF MATERIALS CHEMISTRY B. 8:2040-2047.Full Text via DOI: 10.1039/c9tb01818b Web of Science: 000520502600018
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2020Flavonoid GL-V9 induces apoptosis and inhibits glycolysis of breast cancer via disrupting GSK-3 beta-modulated mitochondrial binding of HKII. FREE RADICAL BIOLOGY AND MEDICINE. 146:119-129.Full Text via DOI: 10.1016/j.freeradbiomed.2019.10.413 Web of Science: 000504866900010
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2019LZ-101, a novel derivative of danofloxacin, induces mitochondrial apoptosis by stabilizing FOXO3a via blocking autophagy flux in NSCLC cells. CELL DEATH & DISEASE. 10.Full Text via DOI: 10.1038/s41419-019-1714-y Web of Science: 000472439500001
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2019AmyZ1: a novel alpha-amylase from marine bacterium Pontibacillus sp. ZY with high activity toward raw starches. BIOTECHNOLOGY FOR BIOFUELS. 12.Full Text via DOI: 10.1186/s13068-019-1432-9 Web of Science: 000465384500002
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2019Enhancing the catalytic performance of chloroperoxidase by co-immobilization with glucose oxidase on magnetic graphene oxide. BIOCHEMICAL ENGINEERING JOURNAL. 143:101-109.Full Text via DOI: 10.1016/j.bej.2018.12.013 Web of Science: 000460809900012
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2017Bioconversion of non-steroidal anti-inflammatory drugs diclofenac and naproxen by chloroperoxidase. BIOCHEMICAL ENGINEERING JOURNAL. 120:7-16.Full Text via DOI: 10.1016/j.bej.2016.12.018 Web of Science: 000395603900002
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2017PLSCR1/IP3R1/Ca2+ axis contributes to differentiation of primary AML cells induced by wogonoside. CELL DEATH & DISEASE. 8.
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2017Pseudoruegeria marinistellae sp nov., isolated from an unidentified starfish in Sanya, China. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY. 110:187-194.
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2016A cold-adapted and glucose-stimulated type II alpha-glucosidase from a deep-sea bacterium Pseudoalteromonas sp K8. BIOTECHNOLOGY LETTERS. 38:345-349.
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2016A novel ethanol-tolerant laccase, Tvlac, from Trametes versicolor. BIOTECHNOLOGY LETTERS. 38:471-476.
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2016Improve ethanol tolerance of beta-glucosidase Bgl1A by semi-rational engineering for the hydrolysis of soybean isoflavone glycosides. JOURNAL OF BIOTECHNOLOGY. 227:64-71.
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2016Lutimaribacter marinistellae sp nov., isolated from a starfish. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 66:3675-3680.
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2016Oroxylin A regulates glucose metabolism in response to hypoxic stress with the involvement of Hypoxia-inducible factor-1 in human hepatoma HepG2 cells. MOLECULAR CARCINOGENESIS. 55:1275-1289.
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2016PspAG97A: A Halophilic alpha-Glucoside Hydrolase with Wide Substrate Specificity from Glycoside Hydrolase Family 97. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY. 26:1933-1942.
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2016Spectroscopic and QM/MM investigations of Chloroperoxidase catalyzed degradation of orange G. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS. 596:1-9.
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2016Structures of PspAG97A alpha-glucoside hydrolase reveal a novel mechanism for chloride induced activation. JOURNAL OF STRUCTURAL BIOLOGY. 196:426-436.
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2015A mechanism of glucose tolerance and stimulation of GH1 beta-glucosidases. SCIENTIFIC REPORTS. 5.
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2015Genome sequence of a laccase producing fungus Trametes sp AH28-2. JOURNAL OF BIOTECHNOLOGY. 216:167-168.
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2015Identification of a laccase Glac15 from Ganoderma lucidum 77002 and its application in bioethanol production. BIOTECHNOLOGY FOR BIOFUELS. 8.
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2015Oroxylin A promotes PTEN-mediated negative regulation of MDM2 transcription via SIRT3-mediated deacetylation to stabilize p53 and inhibit glycolysis in wt-p53 cancer cells. JOURNAL OF HEMATOLOGY & ONCOLOGY. 8.
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2014Characterization of a Novel beta-Glucosidase from Gongronella sp W5 and Its Application in the Hydrolysis of Soybean Isoflavone Glycosides. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 62:11688-11695.
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2014Oroxylin A has therapeutic potential in acute myelogenous leukemia by dual effects targeting PPAR gamma and RXR alpha. INTERNATIONAL JOURNAL OF CANCER. 134:1195-1206.
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2014Wogonin induces cell cycle arrest and erythroid differentiation in imatinib-resistant K562 cells and primary CML cells. ONCOTARGET. 5:8188-8201.
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2012A 3D localized surface plasmon resonance biosensor for the study of trivalent arsenic binding to the ArsA ATPase. BIOSENSORS & BIOELECTRONICS. 38:19-26.
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2012Beclin 1-mediated autophagy in hepatocellular carcinoma cells: Implication in anticancer efficiency of oroxylin A via inhibition of mTOR signaling. CELLULAR SIGNALLING. 24:1722-1732.
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2012Gambogic acid promotes apoptosis and resistance to metastatic potential in MDA-MB-231 human breast carcinoma cells. BIOCHEMISTRY AND CELL BIOLOGY. 90:718-730.
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2012HIF-1 alpha is critical for hypoxia-mediated maintenance of glioblastoma stem cells by activating Notch signaling pathway. CELL DEATH AND DIFFERENTIATION. 19:284-294.
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2012The structures of three metabolites of the algal hepatotoxin okadaic acid produced by oxidation with human cytochrome P450. BIOORGANIC & MEDICINAL CHEMISTRY. 20:3742-3745.
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2009Isolation and characterization of cancer stem like cells in human glioblastoma cell lines. CANCER LETTERS. 279:13-21.
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2009MAC related mitochondrial pathway in oroxylin A induces apoptosis in human hepatocellular carcinoma HepG2 cells. CANCER LETTERS. 284:198-207.
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2009Speciation, formation, stability and analytical challenges of human arsenic metabolites. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY. 24:1397-1405.
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2008Anti-invasive effect of gambogic acid in MDA-MB-231 human breast carcinoma cells. BIOCHEMISTRY AND CELL BIOLOGY. 86:386-395.
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2008Gambogic acid induced tumor cell apoptosis by T lymphocyte activation in H-22 transplanted mice. INTERNATIONAL IMMUNOPHARMACOLOGY. 8:1493-1502.
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2008Gambogic acid mediates apoptosis as a p53 inducer through down-regulation of mdm2 in wild-type p53-expressing cancer cells. MOLECULAR CANCER THERAPEUTICS. 7:3298-3305.
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2008Inhibition of glioblastoma growth and angiogenesis by garnbogic acid: An in vitro and in vivo study. BIOCHEMICAL PHARMACOLOGY. 75:1083-1092.
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2008Involvement of matrix metalloproteinase 2 and 9 in gambogic acid induced suppression of MDA-MB-435 human breast carcinoma cell lung metastasis. JOURNAL OF MOLECULAR MEDICINE-JMM. 86:1367-1377.
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2008Oroxylin A induces G(2)/M phase cell-cycle arrest via inhibiting Cdk7-mediated expression of Cdc2/p34 in human gastric carcinoma BGC-823 cells. JOURNAL OF PHARMACY AND PHARMACOLOGY. 60:1459-1463.
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2008Posttranscriptional regulation of the telomerase hTERT by gambogic acid in human gastric carcinoma 823 cells. CANCER LETTERS. 262:223-231.
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2008Wogonin induced differentiation and G1 phase arrest of human U-937 leukemia cells via PKC delta phosphorylation. EUROPEAN JOURNAL OF PHARMACOLOGY. 591:7-12.
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2008Wogonin suppresses tumor growth in vivo and VEGF-induced angiogenesis through inhibiting tyrosine phosphorylation of VEGFR2. LIFE SCIENCES. 82:956-963.
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2007Differential apoptotic induction of gambogic acid, a novel anticancer natural product, on hepatoma cells and normal hepatocytes. CANCER LETTERS. 256:259-266.
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2007Large scale preparation of the mammalian high mobility group protein A2 for biophysical studies. PROTEIN AND PEPTIDE LETTERS. 14:87-91.
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2006Inhibition of human telomerase reverse transcriptase gene expression by gambogic acid in human hepatoma SMMC-7721 cells. LIFE SCIENCES. 78:1238-1245.
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2006Involvement of bax/bcl-2 in wogonin-induced apoptosis of human hepatoma cell line SMMC-7721. ANTI-CANCER DRUGS. 17:797-805.Full Text via DOI: 10.1097/01.cad.0000217431.64118.3f PMID: 16926629 Web of Science: 000240257100007
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2006Oroxylin A induced apoptosis of human hepatocellular carcinoma cell line HepG2 was involved in its antitumor activity. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 351:521-527.
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2006Repression of telomerase reverse transcriptase mRNA and hTERT promoter by gambogic acid in human gastric carcinoma cells. CANCER CHEMOTHERAPY AND PHARMACOLOGY. 58:434-443.
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2006The anticancer activities of wogonin in murine sarcoma S180 both in vitro and in vivo. BIOLOGICAL & PHARMACEUTICAL BULLETIN. 29:1132-1137.
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2005Potential energy surface and product branching ratios for the reaction of F(P-2) with the methyl radical: An ab initio/RRKM study. CHEMICAL PHYSICS LETTERS. 406:60-74.Full Text via DOI: 10.1016/j.cplett.2005.02.081 Web of Science: 000228612200011
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Correction
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2019Oroxylin A promotes PTEN-mediated negative regulation of MDM2 transcription via SIRT3-mediated deacetylation to stabilize p53 and inhibit glycolysis in wt-p53 cancer cells (vol 8, 41, 2015). JOURNAL OF HEMATOLOGY & ONCOLOGY.Full Text via DOI: 10.1186/s13045-019-0792-8 Web of Science: 000510918500001
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Meeting Abstract
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2018Effects of the pro-cys-pro tripeptide stretch on the structural and biochemical properties of chloroperoxidase. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.Web of Science: 000435537701001
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2017Chloroperoxidase oxidative transformation of roxarsone. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.Web of Science: 000430569101264
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2017Elucidating the role of the proximal ligand loop in chloroperoxidase catalysis. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.Web of Science: 000429525601346
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2015Role of proximal thiolate ligand in chloroperoxidase catalysis. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.Web of Science: 000411183300569
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2014Reactivity of chloroperoxidase, a promising bioengineering target, revealed through simulation. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.Web of Science: 000348455204612
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2010Structural Dynamics in Chloroperoxidase: A Photoacoustic Study. BIOPHYSICAL JOURNAL. 643A-643A.Full Text via DOI: 10.1016/j.bpj.2009.12.3523 Web of Science: 000208762006221
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2009Dynamics And Energetics Associated With Ligand Photodissociation From Co Bound Chloroperoxidase. BIOPHYSICAL JOURNAL. 437A-437A.Web of Science: 000426354000332
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2009Relative stability of iron-sulfur bond vs. iron-nitrogen bond in heme thiolate protein: Evidence from an engineered cytochrome P450cam with a novel heme iron ligand set. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.Web of Science: 000207862000778
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2009Single amino acid switch turns P450cam from a monooxygenase to an efficient peroxidase. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.Web of Science: 000207857805748
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2008INOR 523-Engineering P450 into P420 with catalytic activities. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.Web of Science: 000271775106503
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2007Catalytic roles of Phe103 at the substrate-binding pocket of chloroperoxidase: Remote control of heme chemistry in heme-thiolate proteins. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.Web of Science: 000207593909599
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2006INOR 1013-Structure of the green prosthetic group in chloroperoxidase following mechanism-based modification with terminal alkenes. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.Web of Science: 000207781606676
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Proceedings Paper
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2010Analytical challenges of human arsenic metabolites. ARSENIC IN GEOSPHERE AND HUMAN DISEASES. 498-499.Web of Science: 000392030500196
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Review
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2012Engineered Proteins: Redox Properties and Their Applications. ANTIOXIDANTS & REDOX SIGNALING. 1796-1822.
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Works By Students
chaired theses and dissertations
- tan, yi, Enzyme Catalyzed and Ultrasound Assisted Transformation of Selected Pollutants 2017
- He, Qinghao, The Degradation of Pharmaceutical Pollutants in Wastewater Catalyzed by Chloroperoxidase and the Construction of Chloroperoxidase H105R Mutant 2016
- Shersher, Elena, The Influence of the Proximal Thiolate Ligand and Hydrogen Bond Network of the Proximal Helix on the Structural and Biochemical Properties of Chloroperoxidase 2016
- Zhang, Rui, Nuclear Magnetic Resonance Spectroscopic and Computational Investigations of Chloroperoxidase Catalyzed Regio- and Enantio-Selective Transformations 2013
- Jiang, Lin, Mechanisms of Chloroperoxidase-catalyzed Enantioselective Reactions as Probed by Site-directed Mutagenesis and Isotopic Labeling 2012
- Chen, Taiyi, Chloroperoxidase Catalyzed Enantioselective Epoxidation of Selected Olefins and Regiospecific Degradation of Dimethylsulfoniopropionate 2011
- Wang, Zhonghua, Proton Nuclear Magnetic Resonance Investigation of the Native and Modified Active Site Structure of Heme Proteins 2011
- Wang, Zheng, Characterization of Recombinant Chloroperoxidase, and F103A and C29H/C79H/C87H Mutants 2011
- Tian, Hui, Tailoring Heme-Thiolate Proteins into Efficient Biocatalysts with High Specificity and Selectivity 2010
Research
principal investigator on
- Acquisition of an X-Ray/UV photoelectron spectroscopy setup for materials research and education. awarded by U.S. Army 2007 - 2009
- Consortium Agreement on the Metabolism and Mechanism of the Anticancer Effects of Wogonin, Baicalein and their Derivatives awarded by China Pharmaceutical University 2016 - 2022
- Structural Basis for the Chloroperoxidase-Catalyzed Enantioselective Transformations. awarded by National Science Foundation 2005 - 2010
co-principal investigator on
Contact
full name
- Xiaotang Wang
visualizations
Recent publications and grants in Scholars@FIU
publication subject areas
- Agriculture
- Biochemistry & Molecular Biology
- Biophysics
- Biotechnology & Applied Microbiology
- Cell Biology
- Chemistry
- Electrochemistry
- Endocrinology & Metabolism
- Energy & Fuels
- Engineering
- Food Science & Technology
- Genetics & Heredity
- Hematology
- Immunology
- Instruments & Instrumentation
- Microbiology
- Oncology
- Pharmacology & Pharmacy
- Physics
- Research & Experimental Medicine
- Science & Technology - Other Topics
- Spectroscopy