Co-culture with assisted hatching of human embryos using Buffalo rat liver cells Article

Hu, Y, Maxson, W, Hoffman, D et al. (1998). Co-culture with assisted hatching of human embryos using Buffalo rat liver cells . 13(1), 165-168. 10.1093/humrep/13.1.165

cited authors

  • Hu, Y; Maxson, W; Hoffman, D; Ory, S; Eager, S; Dupre, J; Worrilow, K

fiu authors


  • Commercially obtained Buffalo rat liver (BRL) cells were grown in monolayer culture. The effect of BRL cell co-culture with assisted hatching on embryo development, implantation and pregnancy was investigated in a population of 200 'first-time' in-vitro fertilization (IVF) patients, subdivided into three groups according to the methods of fertilization [IVF; intracytoplasmic sperm injection (ICSI); ICSI/IVF]. Assisted hatching was performed on all embryos chosen for transfer. Following co-culture, the overall embryo quality, implantation rate and pregnancy rates were not significantly different from the controls. However, when grouped according to fertilization method, co-culture was found to have an impact on pregnancy and implantation rates in the group undergoing conventional IVF. Using co-culture with assisted hatching, we were able to achieve a 58% (38/65) clinical pregnancy rate with a 49% (32/65) live birth rate and a 26% (60/235) implantation rate. No changes in the pregnancy and implantation rates were apparent in ICSI or ICSI/IVF subgroups. This is the first prospective, randomly controlled study which reports the use of BRL cell co-culture for human IVF for a large number of patients undergoing IVF for the first time.

publication date

  • January 1, 1998

Digital Object Identifier (DOI)

start page

  • 165

end page

  • 168


  • 13


  • 1