Enhanced microfluidic systematic evolution of ligands by exponential enrichment (EM-SELEX) Conference

cited authors

  • Qian, JK; Lou, X; Zhang, Y; Xiao, Y; Gerdon, A; Soh, HT

fiu authors

abstract

  • Systematic Evolution of Ligands by Exponential Enrichment (termed SELEX) is a combinatorial technique for producing high affinity oligonucleotides (aptamers) of either DNA or RNA that specifically bind to a variety of target ligands, including small molecules, proteins, tissues and organisms. In previous work, we demonstrated a high efficient SELEX platform for rapid generation of aptamers through a microfluidic separation device under a high stringency selection condition. In this work, we significantly extend the capability of M-SELEX method from a rapid evolution of high affinity aptamers to a development of high molecular specificity of the selected aptamers via microfluidic negative selection.

publication date

  • January 1, 2008

start page

  • 1450

end page

  • 1452