Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results Article

Møller, P, Azqueta, A, Boutet-Robinet, E et al. (2020). Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results . 15(12), 3817-3826. 10.1038/s41596-020-0398-1



cited authors

  • Møller, P; Azqueta, A; Boutet-Robinet, E; Koppen, G; Bonassi, S; Milić, M; Gajski, G; Costa, S; Teixeira, JP; Costa Pereira, C; Dusinska, M; Godschalk, R; Brunborg, G; Gutzkow, KB; Giovannelli, L; Cooke, MS; Richling, E; Laffon, B; Valdiglesias, V; Basaran, N; Del Bo’, C; Zegura, B; Novak, M; Stopper, H; Vodicka, P; Vodenkova, S; de Andrade, VM; Sramkova, M; Gabelova, A; Collins, A; Langie, SAS

fiu authors

abstract

  • The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between ‘desirable’ and ‘essential’ information: ‘essential’ information refers to the precise details that are necessary to assess the quality of the experimental work, whereas ‘desirable’ information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.

publication date

  • December 1, 2020

Digital Object Identifier (DOI)

start page

  • 3817

end page

  • 3826

volume

  • 15

issue

  • 12