The expression of cytochrome P450IIB1 in saccharomyces cerevisia results in an increased mutation frequency when exposed to cyclophosphamide Article

Black, SM, Ellard, S, Meehan, RR et al. (1989). The expression of cytochrome P450IIB1 in saccharomyces cerevisia results in an increased mutation frequency when exposed to cyclophosphamide . 10(11), 2139-2143. 10.1093/carcin/10.11.2139

cited authors

  • Black, SM; Ellard, S; Meehan, RR; Parry, JM; Adesnik, M; Beggs, JD; Wolf, CR

fiu authors

abstract

  • A recombinant plasmid containing a full length cDNA encoding the rat cytochrome P450IIB1 under the control of the Saccharomyces cerevisiae ADC1 promoter was constructed and transformed into the yeast strain KYI 118. The encoded P450IIB1 protein was produced at a level of between 0.1 and 0.2% of total yeast cellular protein (0.068 nmol/mg total cellular protein). This protein was localized in the mkrosomal fraction and had activity towards the substrate benzyloxy-resorufin, the activity being 0.16 nmol resorufin produced/ min/mg microsomal protein. When exposed to the anticancer drug cyclophosphamide the mutation frequency, as determined by the development of resistance to the arginine analogue canavanine, increased in a dose-dependent manner over a control strain and was up to 16-fold higher at the highest doses used. © 1989 IRL Press.

publication date

  • November 1, 1989

Digital Object Identifier (DOI)

start page

  • 2139

end page

  • 2143

volume

  • 10

issue

  • 11