Fetal cells in the maternal circulation: Isolation by multiparameter flow cytometry and confirmation by polymerase chain reaction Article

Wachtel, S, Elias, S, Price, J et al. (1991). Fetal cells in the maternal circulation: Isolation by multiparameter flow cytometry and confirmation by polymerase chain reaction . 6(10), 1466-1469. 10.1093/oxfordjournals.humrep.a137290

cited authors

  • Wachtel, S; Elias, S; Price, J; Wachtel, G; Phillips, O; Shulman, L; Meyers, C; Simpson, JL; Dockter, M

fiu authors

abstract

  • During pregnancy, nucleated fetal erythrocytes enter the maternal circulation and can be isolated efficiently from the maternal cells by multiparameter flow cytometry. Male DNA, implying presence of a male fetus, can be identified in flowsorted maternal blood by polymerase chain reaction with oligonucleotide primers flanking single-copy Y-specific DNA sequences. Among flow-sorted samples, we correctly identified fetal sex in 17/18 (94%) pregnancies of 10-21 weeks gestation. Maternal blood thus provides a potential opportunity for prenatal diagnosis that could preclude the need for invasive procedures in current use. © 1991 Oxford University Press.

publication date

  • January 1, 1991

start page

  • 1466

end page

  • 1469

volume

  • 6

issue

  • 10