Regulation of platelet-derived growth factor receptor function by integrin-associated cell surface transglutaminase Article

cited authors

  • Zemskov, EA; Loukinova, E; Mikhailenko, I; Coleman, RA; Strickland, DK; Belkin, AM

fiu authors

abstract

  • A functional collaboration between growth factor receptors such as platelet derived growth factor receptor (PDGFR) and integrins is required for effective signal transduction in response to soluble growth factors. However, the mechanisms of synergistic PDGFR/integrin signaling remain poorly understood. Our previous work showed that cell surface tissue transglutaminase (tTG) induces clustering of integrins and amplifies integrin signaling by acting as an integrin binding adhesion co-receptor for fibronectin. Here we report that in fibroblasts tTG enhances PDGFR-integrin association by interacting with PDGFR and bridging the two receptors on the cell surface. The interaction between tTG and PDGFR reduces cellular levels of the receptor by accelerating its turnover. Moreover, the association of PDGFR with tTG causes receptor clustering, increases PDGF binding, promotes adhesion-mediated and growth factor-induced PDGFR activation, and up-regulates downstream signaling. Importantly, tTG is required for efficient PDGF-dependent proliferation and migration of fibroblasts. These results reveal a previously unrecognized role for cell surface tTG in the regulation of the joint PDGFR/integrin signaling and PDGFR-dependent cell responses. © 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

publication date

  • June 12, 2009

Digital Object Identifier (DOI)

start page

  • 16693

end page

  • 16703

volume

  • 284

issue

  • 24