Objective: To investigate the psychophysiological effects of motivational music on 5km run performance. Methods: Thirteen amateur runners were subjected to two random experimental conditions during 5km run. Functional infrared spectroscopy analyses were used to investigate the motivational qualities of music and its activation in the prefrontal cortex. During the proposed exercise, psychophysiological changes (performance, subjective perception of effort and heart rate) were measused for each of the 12.5 laps (400 meters). Results: The chosen songs were able to increase activation in the prefrontal cortex area (over 0.5 ua.|iM positive differences). Motivational music enhanced task performance to a greater degree than the no-music, control condition (Control - 27.02 i 0.35min vs motivational music - 25.31 i 0.3 lmin; improved in 6.33%). A larger effect size was identified during the first laps (Cohen's d) (0.99 - 1st lap; 0.62 - 2nd lap; 0.55 - 3rd lap; 0.61 - 4th lap). Heart rate was higher for motivational music condition, which was considered a derivative response of greater physical work done. Conclusion: The music was able to activate the prefrontal cortex area and alter psychophysiological and performance parameters.