This study analyzed the effects of caffeine ingestion on physical performance and mood state of cyclists in a 1km time trial test. Ten male cyclists performed randomly a 1km time-trial test under two experimental conditions: Caffeine (CAF - 6 mg.kg-1) and placebo (PL) 60 min before the test. During the test were obtained the total time and the mood using the Brunel questionnaire (BRUMS). For comparisons of total time and mood states it was applied the paired Student's t-test and Wilcoxon test, respectively. No significant differences were found in the total time to complete tests and in the mood states between CAF and PL conditions (P>0.05). The results suggested that caffeine does not improve performance or change the mood of cyclists in a 1km time trial test.