Quantitative detection of nitric oxide in exhaled human breath by extractive electrospray ionization mass spectrometry Article

Pan, S, Tian, Y, Li, M et al. (2015). Quantitative detection of nitric oxide in exhaled human breath by extractive electrospray ionization mass spectrometry . 5 10.1038/srep08725

cited authors

  • Pan, S; Tian, Y; Li, M; Zhao, J; Zhu, L; Zhang, W; Gu, H; Wang, H; Shi, J; Fang, X; Li, P; Chen, H

fiu authors

abstract

  • Exhaled nitric oxide (eNO) is a useful biomarker of various physiological conditions, including asthma and other pulmonary diseases. Herein a fast and sensitive analytical method has been developed for the quantitative detection of eNO based on extractive electrospray ionization mass spectrometry (EESI-MS). Exhaled NO molecules selectively reacted with 2-phenyl-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO) reagent, and eNO concentration was derived based on the EESI-MS response of 1-oxyl-2-phenyl-4, 4, 5, 5-tetramethylimidazoline (PTI) product. The method allowed quantification of eNO below ppb level (∼0.02 ppbv) with a relative standard deviation (RSD) of 11.6%. In addition, eNO levels of 20 volunteers were monitored by EESI-MS over the time period of 10 hrs. Long-term eNO response to smoking a cigarette was recorded, and the observed time-dependent profile was discussed. This work extends the application of EESI-MS to small molecules (<30 Da) with low proton affinity and collision-induced dissociation efficiency, which are usually poorly visible by conventional ion trap mass spectrometers. Long-term quantitative profiling of eNO by EESI-MS opens new possibilities for the research of human metabolism and clinical diagnosis.

publication date

  • January 1, 2015

Digital Object Identifier (DOI)

volume

  • 5