Global metabolic profiling using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry Article

Qi, Y, Song, Y, Gu, H et al. (2014). Global metabolic profiling using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry . 1198 15-27. 10.1007/978-1-4939-1258-2_2

cited authors

  • Qi, Y; Song, Y; Gu, H; Fan, G; Chai, Y

fiu authors

abstract

  • Currently, liquid chromatography-mass spectrometry (LC-MS) is one of the most important analytical technologies for detecting hundreds of metabolites in the field of metabolomics. A recent advance in LC that has impacted metabolomics is the development of UPLC (ultra-performance liquid chromatography). In this chapter, we describe the analytical methodologies for the global metabolic profiling of serum, urine, and tissue samples using UPLC-Q-TOF (quadrupole-time-of-flight)-MS. Aqueous metabolites are extracted after adding methanol/acetonitrile/acetone and then analyzed by UPLC-MS under positive and/or negative ionization mode. With the aid of multivariate statistical analysis, separation between various groups can be observed in the score plots, and biomarkers are screened in the loading/ weight/VIP (variable importance in the projection) scatterplots. Furthermore, putative markers can be identified through comparison with the authentic standards based on tandem mass spectrometry (MS/MS) fragmentation pattern and LC retention. We expect that our protocol, with modifications if necessary, can be useful in many metabolomics studies and a wide range of research areas related to small molecules and LC-MS.

publication date

  • January 1, 2014

Digital Object Identifier (DOI)

start page

  • 15

end page

  • 27

volume

  • 1198