A low incidence of p53 mutations in pre-malignant lesions of the oral cavity from non-tobacco users. Other Scholarly Work

Lazarus, P, Garewal, HS, Sciubba, J et al. (1995). A low incidence of p53 mutations in pre-malignant lesions of the oral cavity from non-tobacco users. . 60(4), 458-463. 10.1002/ijc.2910600406

cited authors

  • Lazarus, P; Garewal, HS; Sciubba, J; Zwiebel, N; Calcagnotto, A; Fair, A; Schaefer, S; Richie, JP

fiu authors

abstract

  • To determine the incidence of p53 mutations in pre-malignant lesions of the oral cavity from individuals without prior history of tobacco use, we have analyzed the conserved regions of the p53 gene (exons 5-9) in archival oral cavity lesion specimens obtained from patients with varied tobacco use histories, by polymerase chain reaction/single strand conformational polymorphism (PCR/SSCP) and DNA sequencing analysis. Twenty-six lesions were analyzed from 14 patients, with multiple lesions obtained from 8 patients. Six of these patients used tobacco, (3 being cigarette smokers, 1 ex-cigarette smoker, 1 moderate cigar smoker and 1 snuff chewer). The remaining 8 patients had no prior history of tobacco use. Thirteen of the pre-malignant lesions exhibited severe dysplasia, 9 exhibited moderate dysplasia and 4 exhibited mild dysplasia. Four of the 26 lesions exhibited p53 mutations, each being from a tobacco user. None of the 13 lesions from never-tobacco users exhibited p53 mutations. There was a significantly higher p53 mutation incidence in pre-malignant lesions from tobacco users (including ex-smokers) than in non-tobacco users as well as in cigarette smokers plus snuff chewers than in non-tobacco users. Two of the mutations were observed in lesions exhibiting severe dysplasia: 1 in a lesion exhibiting moderate dysplasia and 1 in a lesion exhibiting mild dysplasia. These data suggest that p53 mutation may be a very early event in oral cavity tumor progression and demonstrate that pre-malignant lesions obtained from non-tobacco users do not exhibit p53 mutations.

publication date

  • February 1, 1995

keywords

  • Adult
  • Aged
  • Aged, 80 and over
  • Base Sequence
  • Cell Transformation, Neoplastic
  • DNA Mutational Analysis
  • Female
  • Genes, p53
  • Humans
  • Leukoplakia, Oral
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Point Mutation
  • Polymerase Chain Reaction
  • Polymorphism, Single-Stranded Conformational
  • Smoking

Digital Object Identifier (DOI)

Medium

  • Print

start page

  • 458

end page

  • 463

volume

  • 60

issue

  • 4