Analysis of SOX2-regulated transcriptome in glioma stem cells Article

Acanda De La Rocha, AM, López-Bertoni, H, Guruceaga, E et al. (2016). Analysis of SOX2-regulated transcriptome in glioma stem cells . 11(9), 10.1371/journal.pone.0163155



cited authors

  • Acanda De La Rocha, AM; López-Bertoni, H; Guruceaga, E; González-Huarriz, M; Martínez-Vélez, N; Xipell, E; Fueyo, J; Gomez-Manzano, C; Alonso, MM

abstract

  • Introduction G oblastoma is the most malignant brain tumor in adults and is associated with poor survival despite multimodal treatments. Glioma stem-like cells (GSCs) are cells functionally defined by their self-renewal potential and the ability to reconstitute the original tumor upon orthotopic implantation. They have been postulated to be the culprit of glioma chemo- and radio-resistance ultimately leading to relapse. Understanding the molecular circuits governing the GSC compartment is essential. SOX2, a critical transcription regulator of embryonic and neural stem cell function, is deregulated in GSCs however; the precise molecular pathways regulated by this gene in GSCs remain poorly understood. Results We performed a genome-wide analysis of SOX2-regulated transcripts in GSCs, using a microarray. We identified a total of 2048 differentially expressed coding transcripts and 261 non-coding transcripts. Cell adhesion and cell-cell signaling are among the most enriched terms using Gene Ontology (GO) classification. The pathways altered after SOX2 down-modulation includes multiple cellular processes such as amino-acid metabolism and intercellular signaling cascades. We also defined and classified the set of non-coding transcripts differentially expressed regulated by SOX2 in GSCs, and validated two of them. Conclusions We present a comprehensive analysis of the transcriptome controlled by SOX2 in GSCs, gaining insights in the understanding of the potential roles of SOX2 in glioblastoma.

publication date

  • September 1, 2016

Digital Object Identifier (DOI)

volume

  • 11

issue

  • 9